SANT-1

Name: SANT-1
Brand: Tocris Bioscience
SKU: 1974

Tocris Bioscience, part of Bio-Techne | Catalog # 1974

Citations (43)

COA / SDS

Inhibitor of hedgehog (Hh) signaling; antagonizes smoothened activity

Key Product Details

Chemical Name:	N-[(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)methylene]-4-(phenylmethyl)-1-piperazinamine
Purity:	≥98%
Molecular Weight:	373.5 kDa

View Full Technical Details for SANT-1

Technical Data
Citations

Biological Activity for SANT-1

SANT-1 is a potent, cell-permeable inhibitor of Sonic hedgehog (Shh) signaling; high affinity antagonist of smoothened activity (K_D = 1.2 nM). Inhibits smoothened agonist effects with an IC₅₀ of 20 nM (in Shh-LIGHT2 cells). Has been used in protocols to generate pancreatic β cells from hPSCs.

For more information about how SANT-1 may be used, see our protocol: Generation of β cells from hPSCs

Technical Data for SANT-1

M. Wt	373.5 kDa
Formula	C₂₃H₂₇N₅
Storage	Store at +4°C
Purity	≥98%
CAS Number	304909-07-7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SANT-1

Solubility

Soluble Soluble to 50 mM in DMSO

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References for SANT-1

References are publications that support the biological activity of the product.

TM Triolo, JQ Matuschek, R Castro-Gut, AH Shilleh, SPM Williams, MS Hansen, K McDaniel, JM Barra, A Michels, HA Russ Stem-Cell-Derived beta-Like Cells with a Functional PTPN2 Knockout Display Increased Immunogenicity Cells, 2022;11(23):. 2022 PMID: 36497105
KF Cogger, A Sinha, F Sarangi, EC McGaugh, D Saunders, C Dorrell, S Mejia-Guer, Y Aghazadeh, JL Rourke, RA Screaton, M Grompe, PR Streeter, AC Powers, M Brissova, T Kislinger, MC Nostro Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors Nat Commun, 2017;8(1):331. 2017 PMID: 28835709
Nostro M, Sarangi F, Yang C, Holland A, Elefanty A, Stanley E, Greiner D, Keller G Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. Stem Cell Reports, 2015;4(4):591-604. 2015 PMID: 25843049
Nefzger CM, Su CT, Fabb SA, Hartley BJ, Beh SJ, Zeng WR, Haynes JM, Pouton CW Lmx1a allows context-specific isolation of progenitors of GABAergic or dopaminergic neurons during neural differentiation of embryonic stem cells. Stem Cells, 2012;30(7):1349-61. 2012 PMID: 22495882
Chen et al. Small molecule modulation of smoothened activity. Proc.Natl.Acad.Sci., 2002;99:14071