sCy5DA

Name: sCy5DA
Brand: Tocris Bioscience
SKU: 7834

Tocris Bioscience, part of Bio-Techne | Catalog # 7834

COA / SDS

FDAA for super-resolution microscopy of bacteria

Key Product Details

Description:	FDAA for super-resolution microscopy of bacteria
Chemical Name:	(R)-2-Ammonio-3-(6-((E)-3,3-dimethyl-5-sulfo-2-((2E,4E)-5-(1,3,3-trimethyl-5-sulfonato-3H-indol-1-ium-2-yl)penta-2,4-dien-1-ylidene)indolin-1-yl)hexanamido)propanoate
Purity:	≥95% (HPLC)
Molecular Weight:	728.88

View Full Technical Details for sCy5DA

Technical Data
Citations

Biological Activity for sCy5DA

sCy5DA is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans in live bacteria. It is efficiently incorporated into the peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into gram-negative C. crescentus. sCy5DA labelled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/665 nm.

Optical Data for sCy5DA

Emission Color	Red
λ_abs	646 nm
λ_em	665 nm
Extinction Coefficient (ε)	197,240 M^-1cm^-1
Cell Permeable	Yes
Application	STORM

Scientific Data Examples for sCy5DA

	*Single molecule localization microscopy (SMLM) image of Vibrio cholerae* cells labeled with 500 μM sCy5DA**
	*Single molecule localization microscopy (SMLM) image of Bacillus subtilis* cells labeled with 100 μM sCy5DA**
	*Single molecule localization microscopy (SMLM) image of Mycobacterium smegmatis* cells labeled with 100 μM sCy5DA**
	*Single molecule localization microscopy (SMLM) image of Staphylococcus aureus* labeled with 100 μM sCy5DA**

Technical Data for sCy5DA

M. Wt	728.88
Formula	C₃₅H₄₄N₄O₉S₂
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	ZSTSSXALPFZNBO-HHHXNRCGSA-N
Smiles	CC1(C)C2=C(C=CC(S(=O)(O)=O)=C2)N(CCCCCC(NC[C@H](C([O-])=O)[NH3+])=O)/C1=C/C=C/C=C/C3=[N+](C4=C(C3(C)C)C=C(S(=O)([O-])=O)C=C4)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for sCy5DA

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	7.29	10

Preparing Stock Solutions for sCy5DA

The following data is based on the product molecular weight 728.88.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	13.72 mL	68.60 mL	137.20 mL
0.5 mM	2.74 mL	13.72 mL	27.44 mL
1 mM	1.37 mL	6.86 mL	13.72 mL
5 mM	0.27 mL	1.37 mL	2.74 mL

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References for sCy5DA

References are publications that support the biological activity of the product.

Zhang Fluorescent D-amino acids for super-resolution microscopy of the bacterial cell wall. ACS Chem.Biol. 2022 PMID: 35994360