sCy5DL-amide

Name: sCy5DL-amide
Brand: Tocris Bioscience
SKU: 7835

Tocris Bioscience, part of Bio-Techne | Catalog # 7835

COA / SDS

FDAA for super-resolution microscopy of bacteria

Key Product Details

Description:	FDAA for super-resolution microscopy of bacteria
Chemical Name:	2-((1E,3E)-5-((E)-1-(6-(((R)-6-Amino-5-ammonio-6-oxohexyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)penta-1,3-dien-1-yl)-1,3,3-trimethyl-3H-indol-1-ium-5-sulfonate
Purity:	≥95% (HPLC)
Molecular Weight:	769.97

View Full Technical Details for sCy5DL-amide

Technical Data
Citations

Biological Activity for sCy5DL-amide

sCy5DL-amide is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into Gram-positive B.subtilis. sCy5DL-amide labeled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/666 nm.

Optical Data for sCy5DL-amide

Emission Color	Red
λ_abs	646 nm
λ_em	666 nm
Extinction Coefficient (ε)	209,200 M^-1cm^-1
Cell Permeable	Yes
Application	STORM

Scientific Data Examples for sCy5DL-amide

	*Single molecule localization microscopy (SMLM) image of Bacillus subtilis* cells labeled with 100 μM sCy5DA-amide**

Technical Data for sCy5DL-amide

M. Wt	769.97
Formula	C₃₈H₅₁N₅O₈S₂
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	KASKHZOOXRBMET-SSEXGKCCSA-N
Smiles	CC1(C2=C(N(/C1=C/C=C/C=C/C3=[N+](C)C4=C(C=C(C=C4)S(=O)([O-])=O)C3(C)C)CCCCCC(NCCCC[C@@H]([NH3+])C(N)=O)=O)C=CC(S(=O)([O-])=O)=C2)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for sCy5DL-amide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	7.7	10

Preparing Stock Solutions for sCy5DL-amide

The following data is based on the product molecular weight 769.97.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	12.99 mL	64.94 mL	129.88 mL
0.5 mM	2.60 mL	12.99 mL	25.98 mL
1 mM	1.30 mL	6.49 mL	12.99 mL
5 mM	0.26 mL	1.30 mL	2.60 mL

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References for sCy5DL-amide

References are publications that support the biological activity of the product.

Zhang Fluorescent D-amino acids for super-resolution microscopy of the bacterial cell wall. ACS Chem.Biol. 2022 PMID: 35994360