SHIN 1

Name: SHIN 1
Brand: Tocris Bioscience
SKU: 6998

Tocris Bioscience, part of Bio-Techne | Catalog # 6998

Citations (2)

COA / SDS

Potent SHMT inhibitor

Key Product Details

Description:	Potent SHMT inhibitor
Chemical Name:	6-Amino-1,4-dihydro-4-[5-(hydroxymethyl)[1,1'-biphenyl]-3-yl]-3-methyl-4-(1-methylethyl)pyrano[2,3-c]pyrazole-5-carbonitrile
Purity:	≥98% (HPLC)
Molecular Weight:	400.48

View Full Technical Details for SHIN 1

Technical Data
Citations

Biological Activity for SHIN 1

SHIN 1 is a potent serine hydroxymethyltransferase (SHMT) inhibitor (IC₅₀ values are 5 and 13 nM at SHMT1 and SHMT2, respectively). Inhibits growth of HCT-116 cells in vitro (IC₅₀ = 870 nM). Unstable in liver microsome assays.

Licensing Information

Sold under license from Princeton University.

Technical Data for SHIN 1

M. Wt	400.48
Formula	C₂₄H₂₄N₄O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2146095-85-2
PubChem ID	134335083
InChi Key	VVVOFJZXKJKHTD-UHFFFAOYSA-N
Smiles	CC(C1(C2=CC(C3=CC=CC=C3)=CC(CO)=C2)C4=C(OC(N)=C1C#N)NN=C4C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SHIN 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	20.02	50

Preparing Stock Solutions for SHIN 1

The following data is based on the product molecular weight 400.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.99 mL	24.97 mL	49.94 mL
2.5 mM	1.00 mL	4.99 mL	9.99 mL
5 mM	0.50 mL	2.50 mL	4.99 mL
25 mM	0.10 mL	0.50 mL	1.00 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for SHIN 1

References are publications that support the biological activity of the product.

Ducker Human SHMT inhibitors reveal defective glycine import as a targetable metabolic vulnerability of diffuse large B-cell lymphoma. Proc.Natl.Acad.Sci.U.S.A. 2017 PMID: 29073064