SIS3

Name: SIS3
Brand: Tocris Bioscience
SKU: 5291

Tocris Bioscience, part of Bio-Techne | Catalog # 5291

Citations (31)

COA / SDS

Selective Smad3 inhibitor; inhibits TGF-βR1 signaling

Key Product Details

Description:	Selective Smad3 inhibitor; inhibits TGF-βR1 signaling
Chemical Name:	1,2,3,4-Tetrahydro-6,7-dimethoxy-2-[(2E)-3-(1-phenyl-1H-pyrrolo[2,3-b]pyridin-3-yl)-1-oxo-2-propenyl]-isoquinoline hydrochloride
Purity:	≥97% (HPLC)
Molecular Weight:	489.99

View Full Technical Details for SIS3

Technical Data
Citations

Biological Activity for SIS3

SIS3 is a selective Smad3 inhibitor; attenuates TGF-β1-dependent Smad3 phosphorylation and DNA binding. Has no effect on Smad2, p38 MAPK, ERK or PI 3-kinase signaling. Inhibits TGF-β1-induced myofibroblast differentiation of dermal fibroblasts. Also inhibits TGF-β2-induced endothelial cell differentiation in iPSCs.

Compound Libraries for SIS3

SIS3 is also offered as part of the Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for SIS3

M. Wt	489.99
Formula	C₂₈H₂₇N₃O₃.HCl
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	521984-48-5
PubChem ID	16079005
InChi Key	CDKIEBFIMCSCBB-CALJPSDSSA-N
Smiles	O=C(N4CCC(C=C(OC)C(OC)=C5)=C5C4)/C=C/C2=C(C3=CC=CC=C3)N(C)C1=NC=CC=C12.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SIS3

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	49	100

Preparing Stock Solutions for SIS3

The following data is based on the product molecular weight 489.99.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.04 mL	10.20 mL	20.41 mL
5 mM	0.41 mL	2.04 mL	4.08 mL
10 mM	0.20 mL	1.02 mL	2.04 mL
50 mM	0.04 mL	0.20 mL	0.41 mL

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References for SIS3

References are publications that support the biological activity of the product.

Di Bernardini Endothelial lineage differentiation from induced pluripotent stem cells is regulated by microRNA-21 and transforming growth factor β2 (TGF-β2) pathways. J.Biol.Chem. 2014 PMID: 24356956
Boudreau Wild-type and mutant p53 differentially regulate NADPH oxidase 4 in TGF-β-mediated migration of human lung and breast epithelial cells. Br.J.Cancer 2014 PMID: 24714748
Jinnin Characterization of SIS3, a novel specific inhibitor of Smad3, and its effect on transforming growth factor-β1-induced extracellular matrix expression. Mol.Pharmacol. 2006 PMID: 16288083