SP 600125

Name: SP 600125
Brand: Tocris Bioscience
SKU: 1496

Tocris Bioscience, part of Bio-Techne | Catalog # 1496

(4)

Citations (395)

COA / SDS

Selective JNK inhibitor

Key Product Details

Chemical Name:	Anthra[1-9-cd]pyrazol-6(2H)-one
Purity:	≥98%
Molecular Weight:	220.23 kDa

View Full Technical Details for SP 600125

Technical Data
Citations / Reviews (4)

Biological Activity for SP 600125

SP 600125 is a selective JNK inhibitor. Competitively and reversibly inhibits JNK1, 2 and 3 (IC₅₀ = 40 - 90 nM) with negligible activity at ERK2, p38β and a range of enzymes. Active in vivo. Exhibits reduced selectivity over other protein kinases under certain conditions. SP 600125 protects renal tubular epithelial cells from ischemia/reperfusion-induced apoptosis. Promotes chondrogenesis of bone mesenchymal stem cells by inhibition of TNF-α-induced inflammation. Adipogenesis from MSCs is also promoted by SP 600125. Essential component of medium for maintaining stem cells in naive pluripotent state.

Technical Data for SP 600125

M. Wt	220.23 kDa
Formula	C₁₄H₈N₂O
Storage	Store at -20°C
Purity	≥98%
CAS Number	129-56-6

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SP 600125

Solubility

Soluble Soluble to 100 mM in DMSO

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References for SP 600125

References are publications that support the biological activity of the product.

S Warrier, M Van der Je, G Duggal, L Tilleman, E Sutherland, J Taelman, M Popovic, S Lierman, S Chuva De S, A Van Soom, L Peelman, F Van Nieuwe, DIM De Coninck, B Menten, P Mestdagh, J Van de Som, D Deforce, P De Sutter, B Heindryckx Direct comparison of distinct naive pluripotent states in human embryonic stem cells Nat Commun, 2017;8(0):15055. 2017 PMID: 28429706
S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response Cell Death Dis, 2017;8(7):e2955. 2017 PMID: 28749468
Burke S, Goff M, Updegraff B, Lu D, Brown P, Minkin S, Biggerstaff J, Zhao L, Karlstad M, Collier J Regulation of the CCL2 Gene in Pancreatic beta-Cells by IL-1beta and Glucocorticoids: Role of MKP-1. PLoS ONE, 2012;7(10):e46986. 2012 PMID: 23056550
M Imbrechts, K De Samblan, K Fierens, E Brisse, J Vandenhaut, T Mitera, C Libert, I Smets, A Goris, C Wouters, P Matthys IFN-? stimulates CpG-induced IL-10 production in B cells via p38 and JNK signalling pathways Eur. J. Immunol., 2018;0(0):. 2018 PMID: 30004580
Subedi A, Park P Autocrine and paracrine modulation of microRNA-155 expression by globular adiponectin in RAW 264.7 macrophages: involvement of MAPK/NF-kappaB pathway. Cytokine, 2013;64(3):638-41. 2013 PMID: 24084329
IN Baranova, ACP Souza, AV Bocharov, TG Vishnyakov, X Hu, BL Vaisman, MJ Amar, Z Chen, AT Remaley, AP Patterson, PST Yuen, RA Star, TL Eggerman Human SR-BII mediates SAA uptake and contributes to SAA pro-inflammatory signaling in vitro and in vivo PLoS ONE, 2017;12(4):e0175824. 2017 PMID: 28423002
Liu Y, Zhang L, Wang C, Roy S, Shen J Purinergic P2Y2 Receptor Control of Tissue Factor Transcription in Human Coronary Artery Endothelial Cells: NEW AP-1 TRANSCRIPTION FACTOR SITE AND NEGATIVE REGULATOR. J Biol Chem, 2016;291(4):1553-63. 2016 PMID: 26631725
Walczynski J, Lyons S, Jones N, Breitwieser W Sensitisation of c-MYC-induced B-lymphoma cells to apoptosis by ATF2. Oncogene, 2014;33(8):1027-36. 2014 PMID: 23416976
Y Kang, Z Ai, K Duan, C Si, Y Wang, Y Zheng, J He, Y Yin, S Zhao, B Niu, X Zhu, L Liu, L Xiang, L Zhang, Y Niu, W Ji, T Li Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys Cell Rep, 2018;25(9):2563-2576.e9. 2018 PMID: 30485820
R Biedro?, A Peru?, S Józefowski CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide PLoS ONE, 2016;11(4):e0153558. 2016 PMID: 27073833
Anton K, Banerjee D, Glod J Macrophage-associated mesenchymal stem cells assume an activated, migratory, pro-inflammatory phenotype with increased IL-6 and CXCL10 secretion. PLoS ONE, 2012;7(4):e35036. 2012 PMID: 22496888
KH Gupta, JW Goldufsky, SJ Wood, NJ Tardi, GS Moorthy, DZ Gilbert, JP Zayas, E Hahm, MM Altintas, J Reiser, SH Shafikhani Apoptosis and Compensatory Proliferation Signaling Are Coupled by CrkI-Containing Microvesicles Dev. Cell, 2017;41(6):674-684.e5. 2017 PMID: 28633020
Lin Y, Zhang B, Liang H, Lu Y, Ai X, Zhang B, Chen X JNK inhibitor SP600125 enhances TGF-beta-induced apoptosis of RBE human cholangiocarcinoma cells in a Smad-dependent manner. Mol Med Rep, 2013;8(6):1623-9. 2013 PMID: 24100678
Ribas VT, Goncalves BS, Linden R, Chiarini LB Activation of c-Jun N-terminal kinase (JNK) during mitosis in retinal progenitor cells. PLoS ONE, 2012;7(4):e34483. 2012 PMID: 22496813
Carmon K, Gong X, Yi J, Thomas A, Liu Q RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling. Proc Natl Acad Sci U S A, 2014;111(13):E1221-9. 2014 PMID: 24639526
T Kobayashi, H Zhang, WWC Tang, N Irie, S Withey, D Klisch, A Sybirna, S Dietmann, DA Contreras, R Webb, C Allegrucci, R Alberio, MA Surani Principles of early human development and germ cell program from conserved model systems Nature, 2017;546(7658):416-420. 2017 PMID: 28607482
Gafni O, Weinberger L, Mansour AA et al. Derivation of novel human ground state naive pluripotent stem cells. Nature 2013-12-12 PMID: 24172903
L De Clerck, J Taelman, M Popovic, S Willems, M Van der Je, B Heindryckx, P De Sutter, H Marks, D Deforce, M Dhaenens Untargeted histone profiling during naive conversion uncovers conserved modification markers between mouse and human Sci Rep, 2019;9(1):17240. 2019 PMID: 31754138
Zhao et al. Activation of JNKs is essential for BMP9-induced osteogenic differentiation of mesenchymal stem cells. BMB Rep., 2013;46:422 PMID: 23977991
Bennett et al. SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase. Proc.Natl.Acad.Sci.U.S.A., 2001;98:13681 PMID: 11717429
Schnabl et al. TAK1/JNK and p38 have opposite effects on rat hepatic stellate cells. Hepatology, 2001;34:953 PMID: 11679966
Wang et al. SP600125, a selective JNK inhibitor, protects ischemic renal injury via suppressing the extrinsic pathways of apoptosis. Life Sci., 2007;80:2067 PMID: 17459422
Ding et al. SP600125 restored TNF-α-induced impaired chondrogenesis in bone mesenchymal stem cells and its antiosteoarthritis effect in mice. Stem Cells Dev., 2021;30:1028 PMID: 34486378
Tominaga et al. Negative regulation of adipogenesis from human mesenchymal stem cells by Jun N-terminal kinase. Biochem.Biophys.Res.Commun., 2005;326:499 PMID: 15582605