SPRi 3

Name: SPRi 3
Brand: Tocris Bioscience
SKU: 6597

Tocris Bioscience, part of Bio-Techne | Catalog # 6597

Citations (1)

COA / SDS

Potent sepiapterin reductase (SPR) inhibitor; attenuates proliferation of CD4+ T cells

Key Product Details

Description:	Potent sepiapterin reductase (SPR) inhibitor; attenuates proliferation of CD4+ T cells
Chemical Name:	N-[2-(5-Hydroxy-2-methyl-1H-indol-3-yl)ethyl]-2-methoxyacetamide
Purity:	≥98% (HPLC)
Molecular Weight:	262.3

View Full Technical Details for SPRi 3

Technical Data
Citations

Biological Activity for SPRi 3

SPRi 3 is a potent sepiapterin reductase (SPR) inhibitor (IC₅₀ = 53 - 74 nM for human SPR). Inhibits BH4 synthesis pathway and attenuates proliferation in naive CD4⁺ T cells. Suppresses proliferation of human effector CD4⁺ T cells following stimulation. Reduces SPR activity in mouse primary cultures of sensory neurons (IC₅₀ = 450 nM). Ameliorates colitis, suppressing the intestinal infiltration of T cells and other immune cells in vivo. Also reduces immune-cell infiltration into mouse lungs. Reduces neuropathic and inflammatory pain in mouse models.

Licensing Information

Sold under license from the Children's Medical Center Corporation.

Compound Libraries for SPRi 3

SPRi 3 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for SPRi 3

M. Wt	262.3
Formula	C₁₄H₁₈N₂O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1292285-54-1
PubChem ID	52911386
InChi Key	YBXBWBBVLXZQBJ-UHFFFAOYSA-N
Smiles	COCC(NCCC1=C(NC2=CC=C(C=C12)O)C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SPRi 3

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	26.23	100
	ethanol	26.23	100

Preparing Stock Solutions for SPRi 3

The following data is based on the product molecular weight 262.3.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.81 mL	19.06 mL	38.12 mL
5 mM	0.76 mL	3.81 mL	7.62 mL
10 mM	0.38 mL	1.91 mL	3.81 mL
50 mM	0.08 mL	0.38 mL	0.76 mL

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References for SPRi 3

References are publications that support the biological activity of the product.

Latremoliere Reduction of neuropathic and inflammatory pain through inhibition of the tetrahydrobiopterin pathway. Neuron 2015 PMID: 26087165
Cronin The metabolite BH4 controls T cell proliferation in autoimmunity and cancer. Nature 2018 PMID: 30405245
Haruki Tetrahydrobiopterin biosynthesis as a potential target of the kynurenine pathway metabolite xanthurenic acid. J.Biol.Chem. 2016 PMID: 26565027