T 5224

Name: T 5224
Brand: Tocris Bioscience
SKU: 7317

Tocris Bioscience, part of Bio-Techne | Catalog # 7317

Citations (1)

COA / SDS

Selective inhibitor of c-Fos/activator protein (AP)-1

Key Product Details

Description:	Selective inhibitor of c-Fos/activator protein (AP)-1
Chemical Name:	5-[4-(Cyclopentyloxy)-2-hydroxybenzoyl]-2-[(2,3-dihydro-3-oxo-1,2-benzisoxazol-6-yl)methoxy]benzenepropanoic acid
Purity:	≥98% (HPLC)
Molecular Weight:	517.53

View Full Technical Details for T 5224

Technical Data
Citations

Biological Activity for T 5224

T 5224 is a selective inhibitor of c-Fos/activator protein (AP)-1. It inhibits the DNA-binding and transactivation activities of AP-1, without affecting the activity of ATF2, C/EBPα, NF-κB/p65, MyoD, and Sp1 in vitro. In a mouse model, T 5224 prevents type II collagen-induced arthritis by reducing inflammatory cytokines (including interleukin 1β) and matrix-degrading MMPs.

Technical Data for T 5224

M. Wt	517.53
Formula	C₂₉H₂₇NO₈
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	530141-72-1
PubChem ID	23626877
InChi Key	DALCQQSLNPLQFZ-UHFFFAOYSA-N
Smiles	O=C(O)CCC1=CC(=CC=C1OCC2=CC=C3C(=O)NOC3=C2)C(=O)C4=CC=C(OC5CCCC5)C=C4O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for T 5224

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	51.75	100

Preparing Stock Solutions for T 5224

The following data is based on the product molecular weight 517.53.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.93 mL	9.66 mL	19.32 mL
5 mM	0.39 mL	1.93 mL	3.86 mL
10 mM	0.19 mL	0.97 mL	1.93 mL
50 mM	0.04 mL	0.19 mL	0.39 mL

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References for T 5224

References are publications that support the biological activity of the product.

Aikawa Treatment of arthritis with a selective inhibitor of c-Fos/activator protein-1. Nat.Biotechnol 2008 PMID: 18587386
Tsuchida Discovery of nonpeptidic small-molecule AP-1 inhibitors: lead hopping based on a three-dimensional pharmacophore model. J.Med.Chem 2006 PMID: 16392794