T3 CLK

Name: T3 CLK
Brand: Tocris Bioscience
SKU: 7570

Tocris Bioscience, part of Bio-Techne | Catalog # 7570

COA / SDS

Potent and selective pan-CLK inhibitor; also inhibits DYRK1A/B

Key Product Details

Description:	Potent and selective pan-CLK inhibitor; also inhibits DYRK1A/B
Chemical Name:	4-[1,1-Dimethyl-2-(4-methyl-1-piperazinyl)-2-oxoethyl]-N-[6-(4-pyridinyl)imidazo[1,2-a]pyridin-2-yl]benzamide
Purity:	≥98% (HPLC)
Molecular Weight:	482.58

View Full Technical Details for T3 CLK

Technical Data
Citations

Biological Activity for T3 CLK

T3 CLK is a potent and selective pan CDC-like kinase (CLK) inhibitor (IC₅₀ values are 0.67 nM, 15 nM, and 110 nM for CLK1, CLK2, and CLK3 protein kinases, respectively). T3 CLK also inhibits DYRK1A/B (IC₅₀ values are 230 nM and 260 nM for DYRK1B and DYRK1A, respectively in cellular assays). Exhibits dose-dependent alternative splicing effects in HCT116 colorectal cancer cells. T3 CLK is cell permeable, stable and non-toxic.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the T3 CLK probe summary on the SGC website.

Technical Data for T3 CLK

M. Wt	482.58
Formula	C₂₈H₃₀N₆O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2109805-56-1
PubChem ID	132585205
InChi Key	IEFFSHLHNYVSEF-UHFFFAOYSA-N
Smiles	O=C(NC=1N=C2C=CC(=CN2C1)C=3C=CN=CC3)C4=CC=C(C=C4)C(C(=O)N5CCN(C)CC5)(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for T3 CLK

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	24.13	50 with gentle warming
	ethanol	4.83	10 with gentle warming

Preparing Stock Solutions for T3 CLK

The following data is based on the product molecular weight 482.58.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.14 mL	20.72 mL	41.44 mL
2.5 mM	0.83 mL	4.14 mL	8.29 mL
5 mM	0.41 mL	2.07 mL	4.14 mL
25 mM	0.08 mL	0.41 mL	0.83 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for T3 CLK

References are publications that support the biological activity of the product.

Funnell CLK-dependent exon recognition and conjoined gene formation revealed with a novel small molecule inhibitor. Nat.Commun. 2017 PMID: 28232751