TFB-TBOA

Name: TFB-TBOA
Brand: Tocris Bioscience
SKU: 2532

Tocris Bioscience, part of Bio-Techne | Catalog # 2532

Citations (86)

COA / SDS

Potent and selective EAAT1 and EAAT2 blocker

Key Product Details

Description:	Potent and selective EAAT1 and EAAT2 blocker
Chemical Name:	(3S)-3-[[3-[[4-(Trifluoromethyl)benzoyl]amino]phenyl]methoxy]-L-aspartic acid
Purity:	≥98% (HPLC)
Molecular Weight:	426.35

View Full Technical Details for TFB-TBOA

Technical Data
Citations

Biological Activity for TFB-TBOA

TFB-TBOA is a potent and selective glial glutamate transporter EAAT1 and EAAT2 inhibitor (IC₅₀ values are 17, 22 and 300 nM for EAAT2, EAAT1 and EAAT3, respectively). Exhibits selectivity for EAAT1 and EAAT2 over EAAT4 and EAAT5, and a wide range of neuronal receptors and transporters. In HEK293 cells expressing human EAAT1, 2, and 3, TFB-TBOA exhibited selectivity for hEAAT1 and hEAAT2 over hEAAT3 (respective IC₅₀ values are 3.6, 10, and 120 nM), while in tsA201 cells expressing rat EAAT4, [³H]-d-Asp uptake was inhibited with an IC₅₀ of 40 nM. Attenuates glutamate-stimulated intracellular Na⁺ elevation in astrocytes in vitro (IC₅₀ = 43 nM). Induces severe convulsions in vivo.

Compound Libraries for TFB-TBOA

TFB-TBOA is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for TFB-TBOA

M. Wt	426.35
Formula	C₁₉H₁₇F₃N₂O₆
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	480439-73-4
PubChem ID	52941382
InChi Key	LPWONNPEPDHEAI-GJZGRUSLSA-N
Smiles	OC([C@@H](N)[C@H](OCC1=CC=CC(NC(C2=CC=C(C(F)(F)F)C=C2)=O)=C1)C(O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for TFB-TBOA

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	21.32	50

Preparing Stock Solutions for TFB-TBOA

The following data is based on the product molecular weight 426.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.69 mL	23.45 mL	46.91 mL
2.5 mM	0.94 mL	4.69 mL	9.38 mL
5 mM	0.47 mL	2.35 mL	4.69 mL
25 mM	0.09 mL	0.47 mL	0.94 mL

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References for TFB-TBOA

References are publications that support the biological activity of the product.

Fu Chemoenzymatic synthesis and pharmacological characterization of functionalized aspartate analogues as novel excitatory amino acid transporter inhibitors. J.Med.Chem. 2018 PMID: 30011368
Bozzo and Chatton Inhibitory effects of (2S, 3S)-3-[3-[4-(trifluoromethyl)benzoylamino]benzyloxy]aspartate (TFB-TBOA) on the astrocytic sodium responses to glutamate. Brain Res. 2010 PMID: 20026319
Shimamoto and Shigeri Elucidation of glutamate transporter functions using selective inhibitors. CNS Agents Med.Chem.
Magi Physical and functional interaction of NCX1 and EAAC1 transporters leading to glutamate-enhanced ATP production in brain mitochondria. PLoS One 2012 PMID: 22479505
Tsukada Effects of a novel glutamate transporter blocker, (2S, 3S)-3-{3-[4-(trifluoromethyl)benzoylamino]benzyloxy}aspartate (TFB-TBOA), on activities of hippocampal neurons. Neuropharmacology 2005 PMID: 15755476