Thiazovivin

Name: Thiazovivin
Brand: Tocris Bioscience
SKU: 3845

Tocris Bioscience, part of Bio-Techne | Catalog # 3845

Citations (63)

COA / SDS

ROCK inhibitor; improves the efficiency of fibroblast reprogramming and induction of iPSCs

Key Product Details

Description:	ROCK inhibitor; improves the efficiency of fibroblast reprogramming and induction of iPSCs
Chemical Name:	N-Benzyl-[2-(pyrimidin-4-yl)amino]thiazole-4-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	311.36

View Full Technical Details for Thiazovivin

Technical Data
Citations

Biological Activity for Thiazovivin

Thiazovivin is a selective, cell-permeable Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor (IC₅₀ = 0.5 μM). Thiazovivin enhances the efficiency of fibroblast reprogramming to generate induced pluripotent stem cells (iPSCs) when used in combination with SB 431542 (Cat. No. 1614) and PD 0325901 (Cat. No. 4192). Thiazovivin improves the survival of human embryonic stem cells (hESCs) upon trypsinization and increases cell adhesion through the regulation of E-cadherin and significantly improves direct reprogramming efficiency. In combination with Valproic acid, sodium salt (Cat. No. 2815), Purmorphamine (Cat. No. 4551) and A 83-01 (Cat. No. 2939), Thiazovivin can be used to directly reprogram mouse embryonic fibroblasts into chemically induced neural stem cells.

Technical Data for Thiazovivin

M. Wt	311.36
Formula	C₁₅H₁₃N₅OS
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1226056-71-8
PubChem ID	46209426
InChi Key	DOBKQCZBPPCLEG-UHFFFAOYSA-N
Smiles	O=C(NCC3=CC=CC=C3)C1=CSC(NC2=CC=NC=N2)=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Thiazovivin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	31.14	100

Preparing Stock Solutions for Thiazovivin

The following data is based on the product molecular weight 311.36.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.21 mL	16.06 mL	32.12 mL
5 mM	0.64 mL	3.21 mL	6.42 mL
10 mM	0.32 mL	1.61 mL	3.21 mL
50 mM	0.06 mL	0.32 mL	0.64 mL

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References for Thiazovivin

References are publications that support the biological activity of the product.

Xu Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc.Natl.Acad.Sci. USA 2010 PMID: 20406903
Zheng A combination of small molecules directly reprograms mouse fibroblasts into neural stem cells. Biochem.Biophys.Res.Commun. 2016 PMID: 27207831
Lin A chemical platform for improved induction of human iPSCs. Nat.Methods. 2009 PMID: 19838168