Trequinsin hydrochloride

Name: Trequinsin hydrochloride
Brand: Tocris Bioscience
SKU: 2337

Tocris Bioscience, part of Bio-Techne | Catalog # 2337

Citations (4)

COA / SDS

Highly potent PDE3 inhibitor

Key Product Details

Description:	Highly potent PDE3 inhibitor
Alternative Names:	HL-725
Chemical Name:	2,3,6,7-Tetrahydro-9,10-dimethoxy-3-methyl-2-[(2,4,6-trimethylphenyl)imino]-4H-pyrimido[6,1-a]isoquinolin-4-one hydrochloride
Purity:	≥97% (HPLC)
Molecular Weight:	441.95

View Full Technical Details for Trequinsin hydrochloride

Technical Data
Citations

Biological Activity for Trequinsin hydrochloride

Trequinsin hydrochloride is a highly potent inhibitor of cGMP-inhibited phosphodiesterase (PDE3; IC₅₀ = 250 pM). Potently inhibits arachidonic acid-induced aggregation of human platelets (IC₅₀ = 50 pM). Orally active antihypertensive agent; reduces systemic blood pressure in both normotensive and hypertensive animal models. Also activates CatSper channels, increases intracellular Ca²⁺ and cGMP levels, and decreases potassium channel activity in sperm.

Compound Libraries for Trequinsin hydrochloride

Trequinsin hydrochloride is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Trequinsin hydrochloride

M. Wt	441.95
Formula	C₂₄H₂₇N₃O₃.HCl
Storage	Desiccate at RT
Purity	≥97% (HPLC)
CAS Number	78416-81-6
PubChem ID	3060974
InChi Key	DTCZZBVPTHVXFA-UHFFFAOYSA-N
Smiles	Cl.COC1=C(OC)C=C2C(CCN3C(=O)N(C)C(\C=C23)=N\C2=C(C)C=C(C)C=C2C)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Trequinsin hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	44.2	100
	ethanol	44.2	100

Preparing Stock Solutions for Trequinsin hydrochloride

The following data is based on the product molecular weight 441.95.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.26 mL	11.31 mL	22.63 mL
5 mM	0.45 mL	2.26 mL	4.53 mL
10 mM	0.23 mL	1.13 mL	2.26 mL
50 mM	0.05 mL	0.23 mL	0.45 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Trequinsin hydrochloride

References are publications that support the biological activity of the product.

McBrinn Novel pharmacological actions of Trequinsin Hydrochloride improve human sperm cell motility and function. Br.J.Pharmacol. 2019 PMID: 31368510
Lal Trequinsin, a potent new antihypertensive vasodilator in the series of 2-(arylimino)-3-alkyl-9,10-dimethoxy-3,4,6,7-tetrahydro-2H-pyrimido[6,1-a]isoquinolin-4-ones. J.Med.Chem. 1984 PMID: 6492077
Agarwal Role of plasma adenosine in the antiplatelet action of HL 725, a potent inhibitor of cAMP phosphodiesterase: species differences. Thromb.Res. 1987 PMID: 2821650
Ruppert and Weithmann HL 725, an extremely potent inhibitor of platelet phosphodiesterase and induced platelet aggregation in vitro. Life Sci. 1982 PMID: 6294426