Trichostatin A

Name: Trichostatin A
Brand: Tocris Bioscience
SKU: 1406
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1406

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Citations (54)

COA / SDS

Potent histone deacetylase inhibitor

Key Product Details

Description:	Potent histone deacetylase inhibitor
Chemical Name:	(2E,4E,6R)-7-(4-(Dimethylamino)phenyl)-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide
Purity:	≥95% (HPLC)
Molecular Weight:	302.37

View Full Technical Details for Trichostatin A

Technical Data
Citations / Reviews (1)

Biological Activity for Trichostatin A

Trichostatin A is a selective and potent inhibitor of class I and II histone deacetylases (HDAC) (K_i = 3.4 nM, IC₅₀ values are 4.99 nM, 5.21 nM, 16.4 nM, 24.3 nM, 27.6 nM for HDAC1, 3, 6, 10 and 4, respectively). Trichostatin A induces accelerated dedifferentiation of primordial germ cells (PGCs) into embryonic germ (EG) cells. Trichostatin A potently inhibits a range of breast cancer and melanoma cell lines (IC₅₀ = 29-400 nM) and induces apoptosis in prostate cancer cells. In an animal model of Huntington's disease, inhibition of HDAC by Trichostatin A rescues memory deficits and increases expression of CREB/CBP target genes in mutant mice. Activates autophagy, increases vesicular transport of BDNF (Cat. No. 2837) and is active in vivo.Trichostatin A enhances CRISPR-Cas9 gene editing of human induced pluripotent stem cells (iPSCs).

Technical Data for Trichostatin A

M. Wt	302.37
Formula	C₁₇H₂₂N₂O₃
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	58880-19-6
PubChem ID	444732
InChi Key	RTKIYFITIVXBLE-QEQCGCAPSA-N
Smiles	O=C([C@H](C)/C=C(C)/C=C/C(NO)=O)C1=CC=C(N(C)C)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Trichostatin A

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	5

Preparing Stock Solutions for Trichostatin A

The following data is based on the product molecular weight 302.37.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.17 mM	19.45 mL	97.27 mL	194.54 mL
0.85 mM	3.89 mL	19.45 mL	38.91 mL
1.7 mM	1.95 mL	9.73 mL	19.45 mL
8.5 mM	0.39 mL	1.95 mL	3.89 mL

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References for Trichostatin A

References are publications that support the biological activity of the product.

Molugu Trichostatin A for efficient CRISPR-Cas9 gene editing of human pluripotent stem cells. CRISPR J. 2023 PMID: 37676985
Tatamiya Isozyme-selective activity of the HDAC inhibitor MS-275. Proc.Amer.Assoc.Cancer Res.
Giralt Long-term memory deficits in Huntington's disease are associated with reduced CBP histone acetylase activity. Hum.Mol.Genet. 2012 PMID: 22116937
Galluzzi Pharmacological modulation of autophagy: therapeutic potential and persisting obstacles. Nat.Rev.Drug.Discov. 2017 PMID: 28529316
Wharton Inhibition of mitogenesis in Balb/c-3T3 cells by trichostatin A. J.Biol.Chem. 2000 PMID: 10945992
Vigushin Trichostatin A is a histone deacetylase inhibitor with potent antitumor activity against breast cancer in vivo. Clin.Cancer Res. 2001 PMID: 11309348
Yoshida Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A. J.Biol.Chem. 1990 PMID: 2211619
Durcova-Hills Reprogramming primordial germ cells into pluripotent stem cells. PloS ONE 2008 PMID: 18953407