Triptolide

Name: Triptolide
Brand: Tocris Bioscience
SKU: 3253
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 3253

(1)

Citations (36)

COA / SDS

Inhibits RNAPII-mediated transcription; antitumor, anti-inflammatory and immunosuppressive

Key Product Details

Description:	Inhibits RNAPII-mediated transcription; antitumor, anti-inflammatory and immunosuppressive
Chemical Name:	(3bS,4aS,5aS,6R,6aR,7aS,7bS,8aS,8bS)-3b,4,4a,6,6a,7a,7b,8b,9,10-Decahydro-6-hydroxy-8b-methyl-6a-(1-methylethyl)trisoxireno[4b,5:6,7:8a,9]phenanthro[1,2-c]furan-1(3H)-one
Purity:	≥98% (HPLC)
Molecular Weight:	360.4

View Full Technical Details for Triptolide

Technical Data
Citations / Reviews (1)

Biological Activity for Triptolide

Triptolide inhibits DNA-dependent ATPase activity of XBP and induces inhibition of RNA polymerase II (RNAPII)-mediated transcription (IC₅₀ = 200 nM). Selective for RNAPII over RNAPI and RNAPIII. Triptolide blocks RNA synthesis in HeLa cells (IC₅₀ = 62 nM); exhibits potent antiproliferative activity in 60 cancer cell lines (average IC₅₀ = 12 nM) and induces apoptosis by blocking TNF-α-mediated c-IAP1 and c-IAP2 induction. Also displays immunosuppressive and anti-inflammatory activity.

Compound Libraries for Triptolide

Triptolide is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Antiviral Library and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for Triptolide

M. Wt	360.4
Formula	C₂₀H₂₄O₆
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	38748-32-2
PubChem ID	107985
InChi Key	DFBIRQPKNDILPW-CIVMWXNOSA-N
Smiles	O=C1C2=C([C@@](C[C@H]5[C@]4(O5)[C@](O6)3[C@@H]6[C@H]7[C@](C(C)C)(O7)[C@H]4O)([H])[C@]3(C)CC2)CO1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Triptolide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	7.21	20

Preparing Stock Solutions for Triptolide

The following data is based on the product molecular weight 360.4.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	13.87 mL	69.37 mL	138.73 mL
1 mM	2.77 mL	13.87 mL	27.75 mL
2 mM	1.39 mL	6.94 mL	13.87 mL
10 mM	0.28 mL	1.39 mL	2.77 mL

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References for Triptolide

References are publications that support the biological activity of the product.

Lee PG490 (Triptolide) cooperates with tumor necrosis factor-α to induce apoptosis in tumor cells. J.Biol.Chem. 1999 PMID: 10224110
Titov XPB, a subunit of TFIIH, is a target of the natural product triptolide. Nat.Chem.Biol. 2011 PMID: 21278739
Qui Immunosuppressant PG490 (Triptolide) inhibits T-cell interleukin-2 expression at the level of purine-box/nuclear factor of activated T-cells and NF-κB transcriptional activation. J.Biol.Chem. 1999 PMID: 10224109