U0126

Name: U0126
Brand: Tocris Bioscience
SKU: 1144
Rating: 5 (4 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1144

(4)

Citations (609)

COA / SDS

Potent, selective inhibitor of MEK1 and 2

Key Product Details

Description:	Potent, selective inhibitor of MEK1 and 2
Chemical Name:	1,4-Diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene
Molecular Weight:	380.48

View Full Technical Details for U0126

Technical Data
Citations / Reviews (4)

Biological Activity for U0126

U0126 is a potent and selective non-competitive inhibitor of MAP kinase kinase. Inhibits MEK-1 and MEK-2 (IC₅₀ values of 0.07 and 0.06 μM respectively) with little or no effect on the activities of PKC, Abl, Raf, MEKK, ERK, JNK, MKK-3, MKK-4/SEK, MKK-6, Cdk2 or Cdk4. Brain penetrant. Helps maintain human pluripotent stem cells in undifferentiated state.

Negative Control also available.

Technical Data for U0126

M. Wt	380.48
Formula	C₁₈H₁₆N₆S₂
Storage	Desiccate at +4°C
CAS Number	109511-58-2
PubChem ID	3006531
InChi Key	DVEXZJFMOKTQEZ-JYFOCSDGSA-N
Smiles	N\C(SC1=CC=CC=C1N)=C(C#N)/C(C#N)=C(\N)SC1=CC=CC=C1N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for U0126

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	38.05	100

Preparing Stock Solutions for U0126

The following data is based on the product molecular weight 380.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.63 mL	13.14 mL	26.28 mL
5 mM	0.53 mL	2.63 mL	5.26 mL
10 mM	0.26 mL	1.31 mL	2.63 mL
50 mM	0.05 mL	0.26 mL	0.53 mL

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Reconstitution Calculator

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References for U0126

References are publications that support the biological activity of the product.

Kinehara Protein kinase C regulates human pluripotent stem cell self-renewal. PLoS One 2013 PMID: 23349801
Namura Intravenous administraton of MEK inhibitor U0126 affords brain protection against forebrain ischemia and focal cerebral ischemia. Proc.Natl.Acad.Sci.U.S.A. 2001 PMID: 11504919
Duncia MEK inhibitors: the chemistry and biological activity of U0126, its analogs, and cyclization products. Bioorg.Med.Chem.Lett. 1998 PMID: 9873633
Favata Identification of a novel inhibitor of mitogen-activated protein kinase kinase. J.Biol.Chem. 1998 PMID: 9660836
Davies Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem.J. 2000 PMID: 10998351