UNC 6934

Name: UNC 6934
Brand: Tocris Bioscience
SKU: 7183

Tocris Bioscience, part of Bio-Techne | Catalog # 7183

Citations (1)

COA / SDS

Antagonizes PWWP1 interaction with nucleosomal H3K36me2, alters NSD2 nucleolar localization

Key Product Details

Description:	Antagonizes PWWP1 interaction with nucleosomal H3K36me2, alters NSD2 nucleolar localization
Chemical Name:	N-Cyclopropyl-3,4-dihydro-3-oxo-N-[[4-[(4-pyrimidinylamino)carbonyl]phenyl]methyl]-2H-1,4-benzoxazine-7-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	443.46

View Full Technical Details for UNC 6934

Technical Data
Citations

Biological Activity for UNC 6934

UNC 6934 binds to the N-terminal proline-tryptophan-tryptophan-proline (PWWP) domain of nuclear receptor-binding SET domain-containing 2 (NSD2). UNC 6934 inhibits PWWP1 interaction with nucleosomal H3K36me2 (IC₅₀ = 104 nM) by occupying the canonical H3K36me2-binding pocket of PWWP1. It selectively binds endogenous NSD2 in cells and causes accumulation of endogenous NSD2 in the nucleolus.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the UNC 6934 probe summary on the SGC website.

Technical Data for UNC 6934

M. Wt	443.46
Formula	C₂₄H₂₁N₅O₄
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2561494-77-5
PubChem ID	146676966
InChi Key	KOZGEDUWAQFVAV-UHFFFAOYSA-N
Smiles	O=C(C1=CC=C(N2)C(OCC2=O)=C1)N(C3CC3)CC4=CC=C(C(NC5=NC=NC=C5)=O)C=C4

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for UNC 6934

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	8.87	20

Preparing Stock Solutions for UNC 6934

The following data is based on the product molecular weight 443.46.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	11.27 mL	56.37 mL	112.75 mL
1 mM	2.25 mL	11.27 mL	22.55 mL
2 mM	1.13 mL	5.64 mL	11.27 mL
10 mM	0.23 mL	1.13 mL	2.25 mL

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References for UNC 6934

References are publications that support the biological activity of the product.

Sankaran A PWWP domain of histone-lysine N-methyltransferase NSD2 binds to dimethylated Lys-36 of histone H3 and regulates NSD2 function at chromatin. J.Biol.Chem. 2016 PMID: 26912663
Dilworth A chemical probe targeting the PWWP domain alters NSD2 nucleolar localization. Nat.Chem.Biol. 2022 PMID: 34782742