Withaferin A

Name: Withaferin A
Brand: Tocris Bioscience
SKU: 2816
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 2816

(2)

Citations (25)

COA / SDS

Inhibits NF-κB activation

Key Product Details

Description:	Inhibits NF-κB activation
Chemical Name:	(4β,5β,6β,22R)-5,6-Epoxy-4,22,27-trihydroxy-1-oxoergosta-2,24-dien-26-oic acid δ-lactone
Purity:	≥98% (HPLC)
Molecular Weight:	470.6

View Full Technical Details for Withaferin A

Technical Data
Citations / Reviews (2)

Biological Activity for Withaferin A

Withaferin A is a steroid lactone that displays anti-inflammatory, antitumor and antiangiogenic activity. Withaferin A inhibits endothelial cells (HUVEC) sprouting in vitro (IC₅₀ = 12 nM) and in vivo, prevents NF-κB activation by inhibiting activation of IKKβ and inhibits chymotrypsin-like activity of the 20S proteasome. In an animal model of Huntington's disease (HD), Withaferin A delays disease progression and increases lifespan; in a cellular model it suppresses aggregation of mutant huntingtin, activates HSF1 and induces Hsp70 chaperone expression.

Technical Data for Withaferin A

M. Wt	470.6
Formula	C₂₈H₃₈O₆
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	5119-48-2
PubChem ID	45489105
InChi Key	DBRXOUCRJQVYJQ-WDBPBROTSA-N
Smiles	O=C1[C@@]2(C)[C@@]([C@H]5C[C@]3([H])C2CC[C@@]4(C)[C@]([H])3CC[C@@H]4[C@@H]([C@]6([H])OC(C(CO)=C(C)C6)=O)C)(O5)[C@@H](O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Withaferin A

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	4.71	10

Preparing Stock Solutions for Withaferin A

The following data is based on the product molecular weight 470.6.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	21.25 mL	106.25 mL	212.49 mL
0.5 mM	4.25 mL	21.25 mL	42.50 mL
1 mM	2.12 mL	10.62 mL	21.25 mL
5 mM	0.42 mL	2.12 mL	4.25 mL

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References for Withaferin A

References are publications that support the biological activity of the product.

Joshi Withaferin A induces heat shock response and ameliorates disease progression in a mouse model of Huntington's disease. Mol.Neurobiol. 2021 PMID: 33904021
Yang The tumor proteasome is a primary target for the natural anticancer compound withaferin A isolated from "Indian Winter Cherry". Mol.Pharmacol. 2007 PMID: 17093135
Kaileh Withaferin A strongly elicits IkB kinase β hyperphosphorylation concomitant with potent inhibition of its kinase activity. J.Biol.Chem. 2007 PMID: 17150968
Mohan Withaferin A is a potent inhibitor of angiogenesis. Angiogenesis 2004 PMID: 15516832