xStAx-VHLL

Name: xStAx-VHLL
Brand: Tocris Bioscience
SKU: 7298

Tocris Bioscience, part of Bio-Techne | Catalog # 7298

COA / SDS

Selective peptide-based β-catenin Degrader (PROTAC®)

Key Product Details

Description:	Selective peptide-based β-catenin Degrader (PROTAC^®)
Purity:	≥95% (HPLC)
Molecular Weight:	3231.89

View Full Technical Details for xStAx-VHLL

Technical Data
Citations

Biological Activity for xStAx-VHLL

xStAx-VHLL is a selective peptide-based β-catenin Degrader (PROTAC^®). Comprises a β-catenin-targeted stapled peptide, xStAx, linked to a VHL-binding peptide. Reduces β-catenin levels in HEK293T cells and colorectal cancer cell lines. Selectively degrades β-catenin over other Wnt signaling pathway components. Inhibits tumor growth in APC^min/+ mice, with constitutively active Wnt signaling, and reduces survival of patient-derived colorectal cancer cell organoids.

β-catenin antibodies validated for Simple Western™ (automated Western) instruments and Western Blot also available: Catalog # AF1329 and NBP1-54467.

PROTAC^® is a registered trademark of Arvinas Operations, Inc., and is used under license.

Technical Data for xStAx-VHLL

M. Wt	3231.89
Formula	C₁₅₄H₂₄₄N₄₈O₂₉
Single Letter Sequence	RRWPRXILDXHVRRVWRALAPYIP (Modifications: Arg-1 = N-terminal Ac, X = (S)-2-(4-pentenyl)alanine, X-6 and X-10 stapled together with a double bond, Arg-17 = Arg-Ahx, Pro-24 = C-terminal amide)
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	IAIGOXKADOABIF-BDNJFVESSA-N
Smiles	O=C(N[C@H](C(N[C@H](C(N1CCC[C@H]1C(N[C@H](C(N[C@](C)(C(N[C@H](C(N[C@H](C(N[C@H]2CC(O)=O)=O)CC(C)C)=O)[C@H](CC)C)=O)CCC/C=C\CCC[C@@](C)(C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(NCCCCCC(N[C@H](C(N[C@H](C(N[C@H](C(N3[C@@H](CCC3)C(N[C@H](C(N[C@H](C(N4[C@@H](CCC4)C(N)=O)=O)[C@H](CC)C)=O)CC5=CC=C(O)C=C5)=O)=O)C)=O)CC(C)C)=O)C)=O)=O)CCCNC(N)=N)=O)CC6=CNC7=C6C=CC=C7)=O)C(C)C)=O)CCCNC(N)=N)=O)CCCNC(N)=N)=O)C(C)C)=O)CC8=CN=CN8)=O)NC2=O)=O)CCCNC(N)=N)=O)=O)CC9=CNC%10=C9C=CC=C%10)=O)CCCNC(N)=N)[C@@H](NC(C)=O)CCCNC(N)=N.O=C(N[C@H](C(N[C@H](C(N%11CCC[C@H]%11C(N[C@H](C(N[C@](C)(C(N[C@H](C(N[C@H](C(N[C@H]%12CC(O)=O)=O)CC(C)C)=O)[C@H](CC)C)=O)CCC/C=C/CCC[C@@](C)(C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(NCCCCCC(N[C@H](C(N[C@H](C(N[C@H](C(N%13[C@@H](CCC%13)C(N[C@H](C(N[C@H](C(N%14[C@@H](CCC%14)C(N)=O)=O)[C@H](CC)C)=O)CC%15=CC=C(O)C=C%15)=O)=O)C)=O)CC(C)C)=O)C)=O)=O)CCCNC(N)=N)=O)CC%16=CNC%17=C%16C=CC=C%17)=O)C(C)C)=O)CCCNC(N)=N)=O)CCCNC(N)=N)=O)C(C)C)=O)CC%18=CN=CN%18)=O)NC%12=O)=O)CCCNC(N)=N)=O)=O)CC%19=CNC%20=C%19C=CC=C%20)=O)CCCNC(N)=N)[C@@H](NC(C)=O)CCCNC(N)=N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for xStAx-VHLL

Solubility

Soluble to 1mg/ml in water

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References for xStAx-VHLL

References are publications that support the biological activity of the product.

Liao A PROTAC peptide induces durable β-catenin degradation and suppresses Wnt-dependent intestinal cancer. Cell Discov. 2020 PMID: 32550000