ZNL 02-096

Name: ZNL 02-096
Brand: Tocris Bioscience
SKU: 7240

Tocris Bioscience, part of Bio-Techne | Catalog # 7240

COA / SDS

Potent and selective Wee1 Degrader (PROTAC®)

Key Product Details

Description:	Potent and selective Wee1 Degrader (PROTAC^®)
Chemical Name:	4-((3-(4-(4-((2-Allyl-1-(6-(2-hydroxypropan-2-yl)pyridin-2-yl)-3-oxo-2,3-dihydro-1H-pyrazolo[3,4-d]pyrimidin-6-yl)amino)phenyl)piperazin-1-yl)propyl)amino)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione
Purity:	≥98% (HPLC)
Molecular Weight:	799.89

View Full Technical Details for ZNL 02-096

Technical Data
Citations

Biological Activity for ZNL 02-096

ZNL 02-096 is a potent and selective Wee1 Degrader (PROTAC^®) that comprises the Wee1 inhibitor AZD 1775 joined by a linker to the cereblon-binding ligand Pomalidomide (Cat. No. 6302). ZNL 02-096 selectively degrades Wee1 at submicromolar concentrations, while sparing PLK1, an AZD 1775 secondary target. In MOLT-4 cells in vitro, ZNL 02-096 induces degradation of Wee1, accumulation of DNA damage, arrest of the cell cycle in the G₂/M phase and apoptosis. The compound shows antiproliferative effects in a panel of 300 cancer cell lines.

Wee1 antibody validated for Western Blot also available: Catalog # NBP1-33506.

PROTAC^® is a registered trademark of Arvinas Operations, Inc., and is used under license.

Licensing Information

Sold under license from Dana-Farber Cancer Institute.

Technical Data for ZNL 02-096

M. Wt	799.89
Formula	C₄₂H₄₅N₁₁O₆
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2414418-49-6
PubChem ID	153555373
InChi Key	LZUDSNUROXNVPH-UHFFFAOYSA-N
Smiles	CC(O)(C1=NC(N2N(C(C3=CN=C(N=C23)NC4=CC=C(N5CCN(CC5)CCCNC6=C7C(N(C(C7=CC=C6)=O)C8CCC(NC8=O)=O)=O)C=C4)=O)CC=C)=CC=C1)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ZNL 02-096

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	39.99	50

Preparing Stock Solutions for ZNL 02-096

The following data is based on the product molecular weight 799.89.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	2.50 mL	12.50 mL	25.00 mL
2.5 mM	0.50 mL	2.50 mL	5.00 mL
5 mM	0.25 mL	1.25 mL	2.50 mL
25 mM	0.05 mL	0.25 mL	0.50 mL

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References for ZNL 02-096

References are publications that support the biological activity of the product.

Li Development and characterization of a Wee1 kinase degrader. Cell Chem.Biol. 2020 PMID: 31735695