VisUBlock Mouse on Mouse Blocking Reagent
R&D Systems, part of Bio-Techne | Catalog # VB001
Key Product Details
Summary for VisUBlock Mouse on Mouse Blocking Reagent
Blocking reagent for use with primary antibodies from mouse host and mouse tissue.
Key Benefits
- Reduces background staining
- Enables staining of mouse tissue with mouse IgG primary antibodies
Reagents Provided
1mL VisUBlock™ Mouse on Mouse Blocking Reagent
Stability and Storage
Store at 2 °C to 8 °C. DO NOT FREEZE.
Precaution
See MSDS.
Reagent Preparation
Mix 30 µL of VisUBlock™ Mouse on Mouse Blocking Reagent with 1mL of preferred buffer such as PBS or TBS. This will produce approximately 30 mL of working solution, which will stain 100-250 tissue sections.
Procedure
- Prior to primary antibody incubation, apply Mouse on Mouse IgG Blocking Reagent to tissue section. Incubate 1 hour at room temperature.
- Tap off, do not rinse, and continue with primary antibody incubation and detection antibody incubation according to protocol set in lab.
Limitations
- FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
- Results may vary due to variations in tissue staining conditions.
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