For Heat Induced Epitope Retrieval of paraffin-embedded tissue samples, what should be the pH of the retrieval buffer?

In house, we most commonly use basic antigen retrieval buffer at pH 9 (Catalog # VCTS021). However, antigen retrieval depends on the tissue and one should always test if antigen retrieval is necessary and also test other antigen retrieval conditions to determine what works best for the specific tissue being stained. R&D Systems offers ready-to-use Antigen Retrieval reagents at various pHs - Catalogs #s VCTS021, VCTS022, and VCTS023.

What is the pH of Acidic Antigen Retrieval reagent (Catalog # VCTS022)?

The Acidic Antigen Retrieval reagent is pH 4.

VisUCyte Antigen Retrieval Reagent-Acidic

Name: VisUCyte Antigen Retrieval Reagent-Acidic
Brand: R&D Systems
SKU: VCTS022

R&D Systems, part of Bio-Techne | Catalog # VCTS022

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Preparation & Storage

Shipping Conditions	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at room temperature. Do not use past expiration date.

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VisUCyte Antigen Retrieval Reagent-Acidic

R&D Systems, part of Bio-Techne | Catalog # VCTS022

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Preparation & Storage

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