3-Aminotyrosine

Name: 3-Aminotyrosine
Brand: Tocris Bioscience
SKU: 7370

Tocris Bioscience, part of Bio-Techne | Catalog # 7370

COA / SDS

Unnatural amino acid for inducing red shift in fluorescent proteins and fluorescent protein-based biosensors

Key Product Details

Description:	Unnatural amino acid for inducing red shift in fluorescent proteins and fluorescent protein-based biosensors
Chemical Name:	3-Amino-L-tyrosine dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	269.12

View Full Technical Details for 3-Aminotyrosine

Technical Data
Citations

Biological Activity for 3-Aminotyrosine

3-Aminotyrosine is an unnatural amino acid that induces red shift when incorporated into fluorescent proteins and fluorescent protein-based biosensors. Causes spontaneous and efficient red shift of GFP in HEK293T cells, shifting excitation and emission maxima by 56 and 95 nm respectively. Induces red shift in a range of fluorescent proteins containing tyrosine-derived chromophores, including teal fluorescent mTFP1, yellow fluorescent Citrine, and circularly permuted YFP and GFP. Also induces red-shift in fluorescent protein-based biosensors, including iGluSnFR (a glutamate sensor), iGABASnFR (a GABA sensor), dLight1.2 (a dopamine sensor) and iATPSnFR1.1 (an ATP sensor).

Technical Data for 3-Aminotyrosine

M. Wt	269.12
Formula	C₉H₁₂N₂O₃.2HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	23279-22-3
PubChem ID	46735196
InChi Key	PIFWBMTWCTUOOQ-KLXURFKVSA-N
Smiles	N[C@H](C(O)=O)CC1=CC(N)=C(C=C1)O.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 3-Aminotyrosine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	26.91	100
	DMSO	26.91	100

Preparing Stock Solutions for 3-Aminotyrosine

The following data is based on the product molecular weight 269.12.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.72 mL	18.58 mL	37.16 mL
5 mM	0.74 mL	3.72 mL	7.43 mL
10 mM	0.37 mL	1.86 mL	3.72 mL
50 mM	0.07 mL	0.37 mL	0.74 mL

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Reconstitution Calculator

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References for 3-Aminotyrosine

References are publications that support the biological activity of the product.

Zhang & Ai A general strategy to red-shift green fluorescent protein-based biosensors. Nat.Chem.Biol. 2020 PMID: 32929278