DBCO PEG4 Biotin

Name: DBCO PEG4 Biotin
Brand: Tocris Bioscience
SKU: 7480

Tocris Bioscience, part of Bio-Techne | Catalog # 7480

COA / SDS

Biotinylation reagent for labeling azide-containing biomolecules via Cu(I)-free click chemistry

Key Product Details

Description:	Biotinylation reagent for labeling azide-containing biomolecules via Cu(I)-free click chemistry
Chemical Name:	(3aS,4S,6aR)-N-[19-(11,12-Didehydrodibenz[b,f]azocin-5(6H)-yl)-15,19-dioxo-3,6,9,12-tetraoxa-16-azanonadec-1-yl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
Purity:	≥95% (HPLC)
Molecular Weight:	749.92

View Full Technical Details for DBCO PEG4 Biotin

Technical Data
Citations

Biological Activity for DBCO PEG4 Biotin

DBCO-PEG4-Biotin is a biotinylation reagent for labeling azide-containing biomolecules via Cu(I)-free Strain-Promoted Alkyne-Azide Click Chemistry (SPAAC) reaction. The hydrophilic PEG4 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. The PEG linker in DBCO-PEG4-Biotin also enhances the accessibility of the biotin moiety, improving the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose.

Technical Data for DBCO PEG4 Biotin

M. Wt	749.92
Formula	C₃₉H₅₁N₅O₈S
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	1255942-07-4
PubChem ID	91757917
InChi Key	LNHSQAOQVNHUGL-QRBHCBQLSA-N
Smiles	C(CCCC(NCCOCCOCCOCCOCCC(NCCC(=O)N1C=2C(C#CC=3C(C1)=CC=CC3)=CC=CC2)=O)=O)[C@H]4[C@@]5([C@](CS4)(NC(=O)N5)[H])[H]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DBCO PEG4 Biotin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	74.99	100
	ethanol	74.99	100

Preparing Stock Solutions for DBCO PEG4 Biotin

The following data is based on the product molecular weight 749.92.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.33 mL	6.67 mL	13.33 mL
5 mM	0.27 mL	1.33 mL	2.67 mL
10 mM	0.13 mL	0.67 mL	1.33 mL
50 mM	0.03 mL	0.13 mL	0.27 mL

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References for DBCO PEG4 Biotin

References are publications that support the biological activity of the product.

Sawant A versatile toolbox for posttranscriptional chemical labeling and imaging of RNA. Nucleic Acids Res. 2016 PMID: 26384420
Bernardim Precise installation of diazo-tagged side-chains on proteins to enable in vitro and in-cell site-specific labeling. Bioconjug.Chem. 2020 PMID: 32375474
Darabedian O-Acetylated chemical reporters of glycosylation can display metabolism-dependent background labeling of proteins but are generally reliable tools for the identification of glycoproteins. Front.Chem. 2020 PMID: 32411667
Agard A comparative study of bioorthogonal reactions with azides. ACS Chem.Biol. 2006 PMID: 17175580