L-Azidohomoalanine hydrochloride

Name: L-Azidohomoalanine hydrochloride
Brand: Tocris Bioscience
SKU: 6584

Tocris Bioscience, part of Bio-Techne | Catalog # 6584

Citations (1)

COA / SDS

Unnatural amino acid for bio-orthogonal labeling of newly synthesized proteins

Key Product Details

Description:	Unnatural amino acid for bio-orthogonal labeling of newly synthesized proteins
Chemical Name:	(S)-2-Amino-4-azidobutanoic acid hydrochloride
Molecular Weight:	180.59

View Full Technical Details for L-Azidohomoalanine hydrochloride

Technical Data
Citations

Biological Activity for L-Azidohomoalanine hydrochloride

L-Azidohomoalanine hydrochloride is an unnatural amino acid. Incorporated into proteins during de novo protein synthesis, with no effect on protein degradation. Requires methionine-free medium for efficient incorporation. Substrate for wild-type methionyl-tRNA synthetase (MetRS) in vivo. Can be used in BONCAT (bio-orthogonal non-canonical amino acid tagging) for the identification of newly synthesized proteins.

Technical Data for L-Azidohomoalanine hydrochloride

M. Wt	180.59
Formula	C₄H₈N₄O₂.HCl
Storage	Store at -20°C
CAS Number	942518-29-8
PubChem ID	147480
InChi Key	NNWQLZWAZSJGLY-VKHMYHEASA-N
Smiles	N[C@H](C(O)=O)CCN=[N+]=[N-].Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for L-Azidohomoalanine hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	18.06	100

Preparing Stock Solutions for L-Azidohomoalanine hydrochloride

The following data is based on the product molecular weight 180.59.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	5.54 mL	27.69 mL	55.37 mL
5 mM	1.11 mL	5.54 mL	11.07 mL
10 mM	0.55 mL	2.77 mL	5.54 mL
50 mM	0.11 mL	0.55 mL	1.11 mL

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Reconstitution Calculator

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References for L-Azidohomoalanine hydrochloride

References are publications that support the biological activity of the product.

Lang & Chin Cellular incorporation of unnatural amino acids and bioorthogonal labeling of proteins. Chem.Rev. 2014 PMID: 24655057
Wang Nonradioactive quantification of autophagic protein degradation with L-azidohomoalanine labeling. Nat.Protoc. 2017 PMID: 28079880
Dieterich Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT). Proc.Natl.Acad.Sci.U.S.A. 2006 PMID: 16769897