MTT

Name: MTT
Brand: Tocris Bioscience
SKU: 5224
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5224

(2)

Citations (9)

COA / SDS

Reagent used in the measurement of cell proliferation

Key Product Details

Description:	Reagent used in the measurement of cell proliferation
Chemical Name:	3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
Purity:	≥98%
Molecular Weight:	414.32

View Full Technical Details for MTT

Technical Data
Citations / Reviews (2)

Biological Activity for MTT

MTT is a reagent used in the measurement of cell proliferation.

Technical Data for MTT

M. Wt	414.32
Formula	C₁₈H₁₆N₅SBr
Storage	Store at +4°C
Purity	≥98%
CAS Number	298-93-1
PubChem ID	64965
InChi Key	AZKSAVLVSZKNRD-UHFFFAOYSA-M
Smiles	CC1=C(C)SC([N+]2=NC(C4=CC=CC=C4)=NN2C3=CC=CC=C3)=N1.[Br-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for MTT

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	PBS	5

Preparing Stock Solutions for MTT

The following data is based on the product molecular weight 414.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.13 mM	18.57 mL	92.83 mL	185.66 mL
0.65 mM	3.71 mL	18.57 mL	37.13 mL
1.3 mM	1.86 mL	9.28 mL	18.57 mL
6.5 mM	0.37 mL	1.86 mL	3.71 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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References for MTT

References are publications that support the biological activity of the product.

S Kim, SK Lee, H Kim, TM Kim Exosomes Secreted from Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Accelerate Skin Cell Proliferation Int J Mol Sci, 2018;19(10):. 2018 PMID: 30314356
Z Zhang, J Wang, J Cheng, X Yu Effects of miR-126 on the STAT3 signaling pathway and the regulation of malignant behavior in lung cancer cells Oncol Lett, 2018;15(6):8412-8416. 2018 PMID: 29805576

MTT