NAI

Name: NAI
Brand: Tocris Bioscience
SKU: 7004

Tocris Bioscience, part of Bio-Techne | Catalog # 7004

Citations (2)

COA / SDS

Reagent for RNA SHAPE-MaP experiments in vivo; cell permeable

Key Product Details

Description:	Reagent for RNA SHAPE-MaP experiments in vivo; cell permeable
Chemical Name:	1H-Imidazol-1-yl(2-methyl-3-pyridinyl)methanone
Molecular Weight:	187.2

View Full Technical Details for NAI

Technical Data
Citations

Biological Activity for NAI

NAI is a reagent for RNA SHAPE-MaP experiments in live cells. Acylates the 2'-OH in RNA, labels single stranded or flexible regions more than folded regions (SHAPE methodology). Enables analysis of RNA secondary structures at single nucleotide resolution. Exhibits greater signals with lower background than 1M7 for in vivo measurement of the RNA structurome. Also more stable than 1M7 in water. β-mercaptoethanol terminates reaction. Cell permeable.

Technical Data for NAI

M. Wt	187.2
Formula	C₁₀H₉N₃O
Storage	Store at -20°C
CAS Number	1055970-47-2
PubChem ID	66563654
InChi Key	OWQPEDNXDCVXJO-UHFFFAOYSA-N
Smiles	CC1=C(C(N2C=CN=C2)=O)C=CC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NAI

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	374.4	2000

Preparing Stock Solutions for NAI

The following data is based on the product molecular weight 187.2.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
20 mM	0.27 mL	1.34 mL	2.67 mL
100 mM	0.05 mL	0.27 mL	0.53 mL
200 mM	0.03 mL	0.13 mL	0.27 mL
1000 mM	0.01 mL	0.03 mL	0.05 mL

Molarity Calculator

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Reconstitution Calculator

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References for NAI

References are publications that support the biological activity of the product.

Kwok Determination of in vivo RNA structure in low-abundance transcripts. Nat.Commun. 2013 PMID: 24336128
Spitale RNA SHAPE analysis in living cells. Nat.Chem.Biol. 2013 PMID: 23178934
Lee Comparison of SHAPE reagents for mapping RNA structures inside living cells. RNA 2017 PMID: 27879433

NAI