TBIA

Name: TBIA
Brand: Tocris Bioscience
SKU: 7788

Tocris Bioscience, part of Bio-Techne | Catalog # 7788

COA / SDS

Bivalent SHAPE cross-linking reagent

Key Product Details

Description:	Bivalent SHAPE cross-linking reagent
Alternative Names:	trans-bis-isatoic anhydride
Chemical Name:	1,6-Dihydrobenzo[1,2-d:4,5-d']bis([1,3]oxazine)-2,4,7,9-tetraone
Purity:	≥95% (HPLC)
Molecular Weight:	248.15

View Full Technical Details for TBIA

Technical Data
Citations

Biological Activity for TBIA

TBIA (trans-bis-isatoic anhydride) is a bifunctional SHAPE (selective 2'-hydroxyl acylation analyzed by primer extension) chemical cross-linking reagent. TBIA has two electrophilic isatoic anhydride moieties, both of which can react with the 2'-OH group in nucleotides adjacent to and distant from the primary RNA sequence. TBIA is used in SHAPE-JuMP (-juxtaposed merged pairs) a technique for exploring RNA tertiary structure proximity on large RNAs, and higher-order RNA-RNA interactions at nanometer resolution.

Technical Data for TBIA

M. Wt	248.15
Formula	C₁₀H₄N₂O₆
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	VKISDEPBWGUMCP-UHFFFAOYSA-N
Smiles	O=C1OC(C2=C(N1)C=C3C(NC(OC3=O)=O)=C2)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for TBIA

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	4.96	20

Preparing Stock Solutions for TBIA

The following data is based on the product molecular weight 248.15.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	20.15 mL	100.75 mL	201.49 mL
1 mM	4.03 mL	20.15 mL	40.30 mL
2 mM	2.01 mL	10.07 mL	20.15 mL
10 mM	0.40 mL	2.01 mL	4.03 mL

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Reconstitution Calculator

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References for TBIA

References are publications that support the biological activity of the product.

Christy Direct mapping of higher-order RNA interactions by SHAPE-JuMP. Biochemistry 2021 PMID: 34121404