Product Features | Clinical Applications | Resources
Gain Pathology Insights with Clear Visualization of RNA Expression
RNAscope’s next-generation ISH chemistry reliably detects gene expression in common pathology scenarios, with improved sensitivity and specificity over traditional ISH technologies.
Why Choose RNAscope™ ISH?
- Superior sensitivity and specificity in FFPE - RNAscope’s next-generation probe design and detection provide exceptional detection of RNA in standard FFPE tissues.
- Visualize RNA expression with unambiguous signal - RNAscope’s robust chemistry produces clear, easily interpreted chromogenic signal compared to traditional ISH technologies.
- Automated protocols to fit your laboratory workflow – With a workflow similar to immunohistochemistry (IHC), and automation on slide stainers, Leica Biosystems and Roche, RNAscope is easily adopted in your laboratory.
- Surpass IHC challenges with probes for any human gene – With probes spanning the transcriptome, RNAscope is ready to identify RNA expression of pathological importance.
RNAscope in Anatomic Pathology
RNAscope in Anatomic Pathology
RNAscope surpasses IHC and traditional ISH in identifying key tumor markers.
RNAscope provides clear, unambiguous signal for:
- Tumor-associated viruses, including HPV, EBV and CMV
- IgKappa and IgLambda in B-cells of lymphoma
- Secreted markers (albumin) or where IHC is inferior (MDM2)
(Image shows Ig Lambda probe detected with RNAscope (brown) in B-cell lymphoma)
RNAscope RNA ISH Diagnostics
RNAscope RNA ISH Diagnostics
RNAscope High Risk HPV probes are automated on the Leica BOND.
- RNAscope ISH Probe High Risk HPV is a CE IVD assay for the detection of 18 high-risk HPV subtypes in oropharyngeal squamous cell carcinoma (OPSCC)
- Automation on the Leica BOND‑III integrates RNAscope into the routine laboratory workflow
(Image shows RNAscope ISH Probe High Risk HPV detected using RNAscope (brown) in OPSCC)
RNAscope in Clinical Development & CDx
RNAscope in Clinical Development & CDx
RNAscope meets the need for biomarker detection and patient selection.
- RNAscope measures predictive markers for patient selection, and is a key tool for pharmacodynamic and biodistribution studies
- Robust performance in clinical use makes RNAscope a trusted assay for patient selection
- ISO 13485 compliance and a global footprint make Bio-Techne a valued IVD partner
(Image shows FGFR3 probe detected with RNAscope (brown) in urothelial carcinoma)
USCAP 2024 Webinar - RNAscope: Current and Emerging Clinical Applications
Key Publications for RNAscope in Anatomic Pathology
- Craig, SG et al. (2019) Recommendations for determining HPV status in patients with oropharyngeal cancers under TNM8 guidelines: a two-tier approach. Br J Canc 120 827-833.
- Guo, L et al. (2018) Ultrasensitive automated RNA in situ hybridization for kappa and lambda light chain mRNA detects B-cell clonality in tissue biopsies with performance comparable or superior to flow cytometry. Mod Pathol 31 385-394.
- Mirghani, H et al. (2015) Diagnosis of HPV-driven head and neck cancer with a single test in routine clinical practice. Mod Pathol. 28 1518-27.
- Schache, AG et al. (2013) Validation of a novel diagnostic standard in HPV-positive oropharyngeal squamous cell carcinoma. Br J Canc 108 1332-9.
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