Concentrating on AAV Impurities with Ultrasensitive Total Protein Detection on Simple Western

Simple Western™ is a multi-attribute method for AAV analysis, characterizing multiple critical quality attributes (CQAs) in one fully automated instrument. Here, we show how AAV identity is monitored with antibodies specific to VP1/VP2/VP3 capsid proteins alongside simultaneous purity measurements using total protein detection with sensitivity that beats gel staining techniques like SYPRO Ruby.