Application Brief 1020: phospho-p27/Kip1 NanoPro Assay

p27, also known as Kip1, is a cell cycle regulatory/inhibitory protein. It is similar to other members of the Cip/Kip" family which includes the p21Cip1/Waf1 and p57Kip2 genes. p27 shares their functional characteristic of being able to bind several different classes of Cyclin and CDK molecules, acting as a CDK inhibitor. We show the response of p27 to EGF treatment in MCF10A cells."