Application Brief 1031: eEF2 NanoPro Assay

Eukaryotic elongation factor-2 (eEF2) catalyzes ribosome translocation during translation of mRNA. Phosphorylation of eEF2 by eEF2 kinase (also known as CaM kinase III) inactivates the protein and can block protein synthesis. Stimulation of growth is associated with a decrease in eEF2 phosphorylation. Inhibition of eEF2 phosphorylation in the hippocampus has recently been associated with an anti-depressant effect. We show that insulin treatment reduces phosphorylation of eEF2 in H4IIE cells. Inhibition of phosphorylation of eEF2 is restored in the presence of Nh125.