Characterization of the protein solubilizer, SimpleSol for imaged capillary isoelectric focusing ( icIEF ) analysis.

In this poster we show that this reagent, SimpleSol can effectively solubilize proteins for icIEF but is a significantly more stable agent than urea, eliminating the need for analysts to prepare urea fresh every time SimpleSol is also stable when pre mixed with methylcellulose as opposed to urea and is compatible with absorbance and native fluorescence detection on Maurice icIEF In addition we show that SimpleSol had less of an impact on the acidic portion of the pH gradient formed during icIEF compared to urea, resulting in more stable pI values of protein peaks relative to using urea.