Comparison of TcBuster™ Non-Viral Transposon System to Lentiviral Transduction in Primary Human T Cells

You can overcome many of the challenges associated with viral gene delivery with TcBuster transposase. TcBuster is a commercially available hyperactive transposase that efficiently integrates multi-cistronic CAR constructs into primary human immune cells. Here, we compared the generation, phenotype, and efficacy of CAR-T cells produced using either TcBuster or lentivirus.

• The TcBuster transposase outperformed lentivirus when inserting larger constructs into primary human T cells.
• Both TcBuster-modified and lentivirus-transduced T cells expanded to full confluency within the G-Rex^® vessel. 
• The population of TcBuster-modified T cells contained a significantly higher percentage of stem cell memory T cells (Tscm) compared to lentiviral-modified cells. 
• αCD19 CAR-T cells generated with either TcBuster or lentivirus were similarly efficacious against CD19 expressing target cells at as low as a 1:4 E:T ratio and over a 48 hour period. 
• Cytokine secretion profiles corroborated the cytotoxicity results and confirmed that CAR-T cell degranulation increases as the E:T ratio increases.