An Engineered IL-2 Enhances T Cell Proliferation without Affecting Exhaustion or Memory Phenotype

The process of using immune cells for cell therapy requires the consistent and robust growth of highly functional cells. To maximize the number of functional immune cells generated by expansion protocols, Bio-Techne has engineered a Recombinant Human IL-2 protein that supports improved T cell expansion, without altering T cell memory or exhaustion marker expression. Download this application note to see how R&D Systems™ new engineered human IL-2 protein compares with the standard IL-2 protein in side-by-side testing using a simple T cell expansion protocol.

Key Takeaways:

• The engineered human IL-2 protein provides increased and sustained growth of CD8⁺/CD4⁺ T cells when compared with the standard IL-2 protein, making it applicable to many T/NK/TIL cell workflows reliant on IL-2 for proliferation.

• While providing increased proliferation capacity, the engineered IL-2 protein does not yield increased exhaustion or more terminally differentiated T cells than the standard IL-2 protein used in this study.