Faster and Easier Charge Heterogeneity Analysis with the iCE 3

Three major usability improvements are now available for the iCE3 system. A new HT cIEF Cartridge increases resolution while reducing run times by eliminating the need for methyl cellulose. A redesigned electrode arm assembly minimizes cathodic drift allowing robust analysis of 100 samples in a batch. The pI calibration and data export processes have been combined into a single automated procedure through the development of enhanced software features. These updates offer greater speed and improved ease of use. In this poster we present the results of these improvements on a model iCE method for analysis of a basic IgG1 mAb. The new HT cartridge reduces analysis time by five minutes, while still providing a highly resolved peak profile comparable to the original FC cartridge. An intermediate precision study demonstrated a %CV of less than 10% for peak all major peak clusters (>5% percent composition). iCE3 Separations Separations were performed on an iCE3 system equipped with either a PrinCE Next µInjector or an Alcott 720 NV Autosampler.