Imaged Capillary Isoelectric Focusing for Analyzing Antibody Drug Conjugates

This whitepaper highlights the use of Imaged Capillary Isoelectric Focusing (icIEF) for analyzing and characterizing antibody-drug conjugates (ADCs), particularly focusing on ImmunoGen's maytansinoid ADCs.

Key Points

• icIEF provides a new method for analyzing ADCs, offering higher resolution, reproducibility, and ease of use compared to traditional methods.
• The charge profiles of ADCs from icIEF complement the drug loading profiles obtained by mass spectrometry, providing valuable information during process and product development.
• icIEF can be used to compare samples in a range of drug-to-antibody ratios, helping to determine the right amount of linker and drug to attain a target drug-to-antibody ratio.