Incorporating RNAscope™ HiPlex Assay into single cell RNA sequencing workflows

Complex, highly heterogeneous tissues, such as the brain, are comprised of multiple cell types and states. However, interrogation of these complex tissue types requires a highly sensitive, specific, and multiplexed spatial approach with single cell resolution. With the RNAscope technology, researchers can:

• Visualize the cellular heterogeneity of complex organs with the multiplexing and spatial capabilities of the RNAscope Multiplex Fluorescent and HiPlex assays
• Detect up to 12 RNA targets simultaneously in the tissue context with the RNAscope HiPlex assay
• Spatially map scRNA-seq gene profiles at the single cell level in the tissue context
• Localize newly identified cell subtypes and cell markers
• Confirm scRNA-seq results from publicly available datasets in the tissue context
• Combine the RNAscope Multiplex Fluorescent Assays with IHC/ IF to simultaneously detect RNA and protein