Multi-Color Flow Cytometry Panels for Immunophenotyping of Expanded T Cells

Application Note Summary

Immune cell therapies using chimeric antigen receptor (CAR) T cells, regulatory T cells, or tumor infiltrating lymphocytes harness the immune system for treating immune disorders and cancer. For CAR-T cell therapy, the process involves isolating T cells, engineering them with CARs to target tumor antigens, expanding them, and reintroducing them into patients for tumor destruction. During this process, monitoring T cell characteristics, such as memory phenotype, activation status, and exhaustion markers, throughout CAR-T cell therapy development is critical to success. Bio-Techne provides an extensive portfolio of ready-to-use conjugated antibodies validated for flow cytometry applications.

In this application note, we demonstrates how our fluorescent-conjugated antibodies can be optimally combined in multicolor flow cytometry panels to monitor the frequency and phenotype of naïve PBMCs and expanded T cells. In combination with Fluorokines™, our conjugated flow cytometry antibodies can effectively ensure efficient monitoring of CAR-T cell frequency and phenotype in immune cell therapy development.

Features of the Multi-Color Flow Cytometry Panels for T Cell Phenotyping App Note include:

• Overview of the immune cell therapy workflow
• Descriptions of our flow cytometry panels for characterizing T cell Activation, Memory, and Exhaustion phenotypes
• Links to our T cell phenotyping antibody panels and Fluorokines for CAR-T detection
• Data showing the use of our multi-color flow panels across several stages of the immune cell therapy development process