RNAscope® Multiplex Fluorescent v2 Assay combined with Immunofluorescence

This Technical Note provides guidelines for performing in situ hybridization (ISH) using an RNAscope® Multiplex Fluorescent Reagent Kit v2 (Cat. No 323100) combined with immunofluorescence (IF) on formalin-fixed paraffin-embedded (FFPE) tissue sections. To detect fluorescent ISH signals, use the RNAscope® Multiplex Fluorescent Kit v2 with the PerkinElmer TSA® Plus System or Opal™ fluorophores. To detect fluorescent immunohistochemistry (IHC), use HRP-conjugated secondary antibody with the PerkinElmer TSA® Plus System or Opal™ fluorophores. For detailed RNAscope® in situ hybridization on FFPE tissue sections and safety guidelines, refer to the RNAscope® Multiplex Fluorescent Reagent v2 User Manual (Doc. No. 323100-USM).