In-Capillary Immunoassay and Total Protein Detection for Adeno-Associated Virus (AAV) Proteins During Purification from Whole-Cell Lysate

During adeno-associated virus (AAV) manufacture, critical quality attributes must be monitored including the presence, identity, and purity of viral vector proteins. Simple Western can be used to study AAV production, and provides speed and sensitivity, completing a run in 3hrs and detecting AAVs >1:200 diluted sample.