Validating Targets for Targeted Protein Degradation Using dTAG - A Comprehensive Workflow Solution

This poster summarizes two case studies illustrating the Bio-Techne dTAG workflow from TAG knock in cell lines for proteins of interest (POI), to different dTAG Degraders available for treatment and subsequent characterization using an automated western blotting platform, Simple Western™. We present data to demonstrate the value of testing both CRBN- and VHL-recruiting dTAG Degraders for each POI target as well as the use of negative control Degraders. We also show the utility of using a FKBP12 antibody for detecting POI dTAG fusion molecules, allowing for a more simplified procedure when probing multiple targets.