Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.
If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.
Single-Cell Western Validated Antibodies
Filter Antibodies: (400)
| Validated Target | Host Species | Vendor / Cat # | Sample | Concentration |
|---|---|---|---|---|
| α-actinin | Goat |
R&D Systems AF8279 |
KPC cells | 100 µg/mL |
| α-actinin4 | Mouse |
Santa Cruz Biotechnology sc-390205 |
HeLa cells | 100 µg/mL |
| α-synuclein | Rabbit |
Cell Signaling Technology 4179 |
SHY5Y cells | 1:10 |
| β-tubulin | Mouse |
Genetex GTX11312 |
Primary Her2+ breast tumor cells | 1:10 |
| β-tubulin* | Rabbit |
Abcam ab6046 |
Murine pancreatic cancer cells | 1:40 |
| β-tubulin* | Mouse |
Genscript A-01717-40 |
Human pluripotent stem cells | 1:40 |
| βIII-tubulin (neuronal) | Mouse |
Sigma Aldrich T8578 |
Human iPSCs | 100 µg/mL |
| Actin | Mouse |
Developmental Studies Hybridoma Bank 224-236-1 |
Dictyostelium discoideum | 100 µg/mL |
| Akt (pan) | Mouse |
Cell Signaling Technology 2920 |
C2C12 cells | 1:10 |
| Akt (pan) | Rabbit |
Cell Signaling Technology 4685 |
C2C12 cells | 1:10 |
| Akt (pan) (C67E7) | Rabbit |
Cell Signaling Technology 4691 |
CRC tumor samples and human cell lines | 1:10 |
| Akt (pan)* | Rabbit |
Cell Signaling Technology 4691 |
C2C12 cells | 1:10 |
| Albumin | Mouse |
Abcam 10241 |
Hepatocyte co-culture | 100 µg/mL |
| Albumin | Rabbit |
Cell Signaling Technology 4929 |
Hepatocyte co-culture | 1:10 |
| ALDH1A1 | Rabbit |
Cell Signaling Technology 12035 |
(unpublished) | 1:10 |
| alpha-actinin | Rabbit |
Cell Signaling Technology 6487 |
BT474 | |
| AML1 | Rabbit |
Cell Signaling Technology 4336 |
Jurkat cells | 1:10 |
| Anti-mouse Alexa Fluor 555 | Goat |
ThermoFisher A-21422 |
CRC tumor samples and human cell lines | 1:20 |
| Anti-mouse Alexa Fluor 647 | Goat |
ThermoFisher A-21235 |
CRC tumor samples and human cell lines | 1:20 |
| Anti-rabbit Alexa Fluor 555 | Goat |
ThermoFisher A-21428 |
CRC tumor samples and human cell lines | 1:20 |
| Anti-rabbit Alexa Fluor 647 | Chicken |
ThermoFisher A-21443 |
CRC tumor samples and human cell lines | 1:20 |
| APRIL | Goat |
R&D Systems AF884 |
MOLT-4 cells | 1:5 |
| ASC | Rabbit |
Adipogen AG-25B-0006-C100 |
GM-CSF BMDCs | 50 µg/mL |
| ATG5 | Rabbit |
Cell Signaling Technology 129945 |
Macrophages | 1:10 |
| Atg7 | Mouse |
R&D Systems MAB6608 |
HeLa cells | 100 µg/mL |
| Atg7* | Rabbit |
Abcam ab133528 |
HeLa cells | 100 µg/mL |
| ATM | Mouse |
Novus Biologicals NB100-309 |
(unpublished) | 100 µg/mL |
| ATP1A3 | Mouse |
Abcam ab2826 |
Live primary human cortical neurons | 100 µg/mL |
| ATP1A3/Sodium Potassium ATPase Alpha 3 | Mouse |
Novus Biologicals NB300-540 |
Live primary human cortical neurons | 100 µg/mL |
| Axl | Rabbit |
Cell Signaling Technology 8661 |
HeLa cells | 1:10 |
| BAFF | Goat |
R&D Systems AF124 |
MOLT-4 cells | 1:10 |
| Bcl-xL | Rabbit |
Cell Signaling Technology 2764 |
HeLa cells | 1:10 |
| BCL6 | Rabbit |
Cell Signaling Technology 5650 |
HeLa cells | 1:10 |
| BCL6 | Rabbit |
Cell Signaling Technology 14895 |
HeLa cells | 1:10 |
| beta-tubulin | Mouse |
Novus Biologicals NBP2-50061 |
Jurkat cells | 50 µg/mL |
| beta-tubulin | Mouse |
Novus Biologicals NB120-7792 |
Jurkat cells | 50 µg/mL |
| betaIII-tubulin | Mouse |
R&D Systems MAB1195 |
neural differentiated iPSCs | 100 µg/mL |
| Blasticidin | Rabbit |
Abcam ab38307 |
Transfected HEK293T cells | 100 µg/mL |
| Blimp-1 | Rabbit |
Cell Signaling Technology 9115 |
U266 cells | 1:10 |
| BRM | Rabbit |
Cell Signaling Technology 11966 |
293T cells | 1:10 |
| BTK | Rabbit |
Cell Signaling Technology 8547 |
Primary sorted human spleen lymphocytes | 1:10 |
| BTK | Mouse |
Cell Signaling Technology 56044 |
Primary sorted human spleen lymphocytes | 1:10 |
| c-Jun | Rabbit |
Cell Signaling Technology 9165 |
KPC cells | 1:10 |
| c-Myc | Rabbit |
Cell Signaling Technology 5605 |
Human pluripotent stem cells | 1:10 |
| Calnexin | Rabbit |
Novus Biologicals NBP1-97476 |
U373 MG cells | 1:10 |
| Calnexin | Rabbit |
Enzo Life Sciences ADI-SPA-865 |
U373 MG cells | 1:10 |
| Cas9 | Mouse |
Abcam ab191468 |
Mouse lymphocytes | 1:10 |
| Caspase 8 | Mouse |
Enzo ADI-AAM-118-E |
Treated Jurkat cells | 1:10 |
| CCL3/MIP1 | Goat |
R&D Systems AF-270-NA |
Differentiated THP-1 treated with LPS and Transport Inhibitors | 20 µg/mL |
| CCR7 | Rabbit |
Novus Biologicals NBP2-58753 |
Jurkat cells | 100 µg/mL |
| CD-80 (B7-1) | Goat |
R&D Systems AF140 |
Dendritic cells | 100 µg/mL |
| CD11b | Rabbit |
Novus Biologicals NB110-89474 |
(unpublished) | 1:10 |
| CD133 (A3G6K) | Rabbit |
Cell Signaling Technology 5860 |
CRC tumor samples and human cell lines | 1:10 |
| CD19 | Rabbit |
Cell Signaling Technology 3574 |
Ramos cells | 1:10 |
| CD209 (DC-SIGN) | Sheep |
R&D Systems AF161 |
Dendritic cells | 100 µg/mL |
| CD25 | Goat |
R&D Systems AF-223-NA |
Tregs | 100 µg/mL |
| CD29 | Goat |
R&D Systems AF2405 |
KPC cells | 100 µg/mL |
| CD3 | Rat |
Cell Signaling Technology 4443 |
MOLT-4 cells | 1:10 |
| CD3e | Rabbit |
ThermoFisher Scientific MA5-14524 |
Differentiated NK cells | 1:10 |
| CD4 | Goat |
R&D Systems AF-379-NA |
PBMCs | 1:10 |
| CD45 | Mouse |
BD Biosciences 555480 |
PBMCs | 100 µg/mL |
| CD45 | Rabbit |
Cell Signaling Technology 13917 |
PBMCs | 1:10 |
| CD56 (NCAM-1) | Goat |
R&D Systems AF2408 |
Differentiated NK cells | 100 µg/mL |
| CD68 | Rat |
Novus Biologicals NBP2-33337 |
RAW264.7 cells | 100 µg/mL |
| CD68/SR-D1 | Rat |
Novus Biologicals NBP2-80656 |
RAW264.7 cells | 100 µg/mL |
| CD8α | Rabbit |
Cell Signaling Technology 85336 |
CD3+ T-cells | 1:10 |
| CD81 | Mouse |
Novus Biologicals NBP2-53318 |
Gli36 cells | 100 µg/mL |
| CD81* | Mouse |
Novus Biologicals NBP1-44861 |
Gli36 cells | 100 µg/mL |
| CD83 | Goat |
R&D Systems AF2044 |
Dendritic cells | 100 µg/mL |
| CD86 (B7-2) | Goat |
R&D Systems AF-141-NA |
Dendritic cells | 100 µg/mL |
| CD90 | Mouse |
Abcam ab23894 |
Cholangiocarcinoma cells | 100 µg/mL |
| cFos | Mouse |
Santa Cruz Biotechnology sc-166940 |
Treated LNCap cells | 20 µg/mL |
| Chromogranin A (CHGA, E8X7R) | Rabbit |
Cell Signaling Technology 85798 |
CRC tumor samples and human cell lines | 1:10 |
| Cleaved caspase 8 | Rabbit |
Cell Signaling Technology 9496 |
Treated Jurkat cells | 1:10 |
| CLIP170 | Mouse |
Santa Cruz Biotechnology sc-166801 |
(unpublished) | 40 µg/mL |
| CLIP170 | Mouse |
Santa Cruz Biotechnology sc-28325 |
(unpublished) | 40 µg/mL |
| CLIP170* | Rabbit |
Cell Signaling Technology 8977 |
(unpublished) | 1:10 |
| Collagen I | Mouse |
Abcam ab6308 |
(unpublished) | 100 µg/mL |
| Cortexillin I | Mouse |
Developmental Studies Hybridoma Bank 241-438-1 |
Dictyostelium discoideum | 1:2 |
| CPEB4 | Rabbit |
Abcam ab1974928 |
(unpublished) | 100 µg/mL |
| CPT1A | Mouse |
Abcam ab128568 |
(unpublished) | 100 µg/mL |
| CRBN | Rabbit |
Novus Biologicals NBP1-91810 |
HeLa cells | 100 µg/mL |
| CRBN | Rabbit |
Cell Signaling Technology 71810 |
HeLa cells | 1:10 |
| CRMP5 | Rat |
Novus Biologicals NB100-74395 |
1:10 | |
| CRMP5 | Rat |
ThermoFisher Scientific MA3-701 |
(unpublished) | 1:10 |
| CYBRD1 | Rabbit |
Genetex GTX104793 |
Red blood cells | 100 µg/mL |
| Cyclin A2 | Rabbit |
Abcam ab181591 |
(unpublished) | 100 µg/mL |
| Cyclin D1 | Rabbit |
R&D Systems AF4196 |
MCF-7 cells | 100 µg/mL |
| Cyclophilin B | Goat |
R&D Systems AF5410 |
HEK293T cells | 100 µg/mL |
| Cytochrome b reductase 1 | Rabbit |
Novus Biologicals NBP2-16075 |
Red blood cells | 100 µg/mL |
| Cytokeratin 14 | Mouse |
ThermoFisher Scientific MA51599 |
normal human mammary basal cells | 1:10 |
| Cytokeratin 14 | Rabbit |
BioLegend 905301 |
Mammary tumor organoid cells | 1:10 |
| Cytokeratin 14 | Rabbit |
ThermoFisher Scientific LBVRB-9020-P0 |
(unpublished) | 1:20 |
| Cytokeratin 19 | Rat |
Developmental Studies Hybridoma Bank TROMA III |
(unpublished) | 50 µg/mL |
| Cytokeratin 19 | Rabbit |
GeneTex GTX112666 |
normal human mammary basal cells | |
| Cytokeratin 8* | Mouse |
Abcam ab9023 |
Breast cancer cells | 100 µg/mL |
| Cytokeratin 8/18* | Rat |
Developmental Studies Hybridoma Bank TROMA-1-S |
Mammary tumor organoid cells | 1:2 |
| DDX4 | Rabbit |
Abcam ab13840 |
(unpublished) | 100 µg/mL |
| DLL4 | Goat |
R&D Systems AF1389 |
KPC cells | 100 µg/mL |
| DNMT3A | Goat |
R&D Systems AF6315 |
SH-SY5Y | 20 µg/mL |
| Dopamine Transporter | Rabbit |
MilliporeSigma AB5802 |
CRC tumor samples and human cell lines | 1:10 |
| E-cadherin | Goat |
R&D Systems AF748 |
KPC cells | 100 µg/mL |
| EDNRA | Mouse |
BD Biosciences 611252 |
(unpublished) | 1:20 |
| Efraim teams | Sheep |
Whole sells products 1234567 |
Products all | 1:30 |
| EGFR | Mouse |
Cell Signaling Technology 2232 |
Circulating tumor cells | 1:10 |
| EGFR E746-A750 | Rabbit |
Cell Signaling Technology 2085 |
HCC827 cells | 1:10 |
| EGFR L858R | Rabbit |
Cell Signaling Technology 3197 |
NCI-H1975 cells | 1:10 |
| EGFR* | Goat |
R&D Systems AF231 |
BEAS-2B cells | 100 µg/mL |
| EGR1 | Rabbit |
Abcam ab133695 |
Treated LNCap cells | 1:20 |
| eIF4E | Rabbit |
Cell Signaling Technology 2067 |
Primary Her2+ breast tumor cells | 1:10 |
| Eos | Rabbit |
EMD Millipore ABE1331 |
HepG2 cells | 100 µg/mL |
| EpCAM | Rabbit |
Cell Signaling Technology 3599 |
BT-20 cells | 1:10 |
| EpCAM | Rabbit |
Abcam ab213500 |
(unpublished) | 100 µg/mL |
| EpCAM* | Goat |
R&D Systems AF960 |
(unpublished) | 100 µg/mL |
| EphB4 | Goat |
R&D Systems AF446 |
Neural stem cells | 1:20 |
| ER | Rabbit |
ThermoFisher Scientific RM-9101-S0 |
MCF-7 cells | 1:10 |
| ERa | Rabbit |
Abcam ab16660 |
MCF-7 cells | 1:10 |
| ERK 1/2 | Mouse |
Cell Signaling Technology 9107 |
A172 cells | 1:10 |
| ERK1/2 | Rabbit |
EMD Millipore 06-182 |
Neural stem cells | 1:20 |
| ERK1/2* | Mouse |
Cell Signaling Technology 4696 |
Stimulated PBMCs | 1:10 |
| ERK1/2* | Rabbit |
Cell Signaling Technology 4695 |
Primary Her2+ breast tumor cells | 1:10 |
| Etv5 | Rabbit |
Abcam ab156221 |
Pedx3-sorted cells | 100 µg/mL |
| FAK | Sheep |
R&D Systems AF4467 |
KPC cells | 100 µg/mL |
| FGFR1 | Rabbit |
Cell Signaling Technology 9740 |
(unpublished) | 1:10 |
| FGFR2 | Rabbit |
Cell Signaling Technology 23328 |
KatoIII cells | 1:10 |
| FKBP5 | Rabbit |
Cell Signaling Technology 12210 |
PBMCs | 1:10 |
| FLAG Tag | Rabbit |
R&D Systems MAB8529 |
3T3 Flag-ATF6 cells | 100 µg/mL |
| FoxL1 | Rabbit |
Abcam ab95286 |
A549 cells | 1:10 |
| FOXP3 | Goat |
R&D Systems BAF3240 |
Mouse T-regs | |
| FOXP3 | Rabbit |
Novus Biologicals NB100-39002 |
FoxP3-transfected HEK293T | 100 µg/mL |
| GAPDH | Goat |
R&D Systems AF5718 |
HT-29 cells | 100 µg/mL |
| GAPDH | Goat |
Novus Biologicals NB300-320 |
Jurkat cells | |
| GAPDH | Mouse |
Novus Biologicals NB300-221 |
freshly isolated flow-sorted cells from skeletal muscle (Ecs, HCs, FAPS, MuSCs), Cells knocked down for TCF4 with siRNA at the level of transcript & protein | 1:40 |
| GAPDH | Goat |
Everest EB06377 |
HeLa-GFP cells | 100 µg/mL |
| GAPDH | Mouse |
Genetex GTX627408 |
U373 MG cells | 1:10 |
| GAPDH | Rabbit |
Cell Signaling Technology 2118 |
HEK293 cells | 1:20 |
| GAPDH | Rabbit |
Cell Signaling Technology 5174 |
HeLa cells | 1:10 |
| GAPDH | Mouse |
EMD Millipore MAB374 |
HeLa cells | 1:20 |
| GAPDH* | Goat |
Sigma Aldrich SAB2500450 |
A431 cells | 1:10 |
| GATA-3 | Mouse |
R&D Systems MAB6330 |
MCF-7 cells | 100 µg/mL |
| GATA-3 | Mouse |
R&D Systems MAB26051 |
MCF-7 cells | 100 µg/mL |
| GATA-3* | Goat |
R&D Systems AF2605 |
MCF-7 cells | 100 µg/mL |
| GATA-4 | Rabbit |
Cell Signaling Technology 36966 |
Cardiomyocytes | 1:10 |
| GDNF | Goat |
R&D Systems AF-212-NA |
U-87 MG | 20 µg/mL |
| GFAP | Sheep |
R&D Systems AF2594 |
Human iPSCs | 1:20 |
| GFP | Mouse |
Sigma Aldrich 11814460001 |
Primary murine germ cells | 100 µg/mL |
| GFP | Rabbit |
ThermoFisher Scientific A-11122 |
Dissociated mouse brain | 100 µg/mL |
| GFP* | Goat |
Abcam ab6673 |
Mouse dendrites | 100 µg/mL |
| Glucagon | Mouse |
EMD Millipore MABN238 |
(unpublished) | 1:10 |
| Glucagon | Rabbit |
Abcam ab108426 |
(unpublished) | 100 µg/mL |
| Glucagon | Mouse |
BD Biosciences 565859 |
(unpublished) | 100 µg/mL |
| Glucocorticoid Receptor | Rabbit |
Cell Signaling Technology 3660 |
PBMCs | 1:10 |
| GPCR MRGX2 | Rabbit |
Novus Biologicals NB110-75035 |
A549 | 1:10 |
| GPR50 | Rabbit |
Cell Signaling Technology 14032 |
293T cells | 1:10 |
| GRP75 | Mouse |
Abcam ab82591 |
U373 MG cells | 1:10 |
| GSK3b | Mouse |
Cell Signaling Technology 9832 |
(unpublished) | 1:10 |
| GST | Rabbit |
Cell Signaling Technology 2625 |
(unpublished) | 1:10 |
| H3K27me3 | Mouse |
Abcam ab205729 |
Human brain nuclei | 100 µg/mL |
| H3K27me3 | Mouse |
Abcam ab6002 |
Treated KARPAS cells | 100 µg/mL |
| H3K79me2 | Rabbit |
Novus Biologicals NBP2-59194 |
HeLa cells | 100 µg/mL |
| H3K79me2* | Rabbit |
Cell Signaling Technology 5427 |
HEK293T cells | 1:10 |
| Helios | Rabbit |
Cell Signaling Technology 42427 |
PBMCs | 1:10 |
| Hemoglobin | Goat |
Genetex GTX77484 |
Red blood cells | 100 µg/mL |
| Hemoglobin* | Mouse |
Rockland Immunochemicals 200-301-GS4 |
Red blood cells | 1:10 (can lower to 1:20) |
| Her2 | Mouse |
ThermoFisher Scientific BMS120FI |
BT474 | |
| Her2 | Mouse |
ThermoFisher Scientific MA513105 |
Primary Her2+ breast tumor cells | 100 µg/mL |
| Her2 | Mouse |
Abcam ab16901 |
BT474 | |
| Hes1 | Rabbit |
Aviva Systems ARP32372-T100 |
(unpublished) | 1:5 |
| HIF1α | Mouse |
BD Biosciences 610958 |
Treated HeLa cells | 50 µg/mL |
| Histone H3 | Rabbit |
Novus Biologicals NB500-171 |
Jurkat cells | |
| Histone H3 | Mouse |
Novus Biologicals NBP2-80770 |
PBMCs | 100 µg/mL |
| Histone H3 | Mouse |
Cell Signaling Technology 3638 |
PBMCS | 1:10 |
| Histone H3 antibody - ChIP grade | Rabbit |
Abcam ab1791 |
CRC tumor samples and human cell lines | 1:50 |
| Histone H3 phospho Ser10 | Rabbit |
Novus Biologicals NBP3-07398 |
CRC tumor samples and human cell lines | 1:30 |
| Histone H3* | Sheep |
Novus Biologicals NB100-747 |
Primary murine germ cells | 100 µg/mL |
| Histone H3* | Mouse |
Novus Biologicals NBP2-36468 |
PBMCs | 100 µg/mL |
| Histone H3* | Rabbit |
Cell Signaling Technology 4499 |
CD4 T-cells | 1:10 |
| HMGB1 | Mouse |
R&D Systems MAB1690 |
HeLa cells | 1:5 |
| HMGCS2 | Rabbit |
Sigma Aldrich SAB2107997 |
(unpublished) | 100 µg/mL |
| HMGCS2* | Rabbit |
Abcam ab137043 |
(unpublished) | 100 µg/mL |
| HSP60 | Mouse |
R&D Systems MAB1800 |
iPSCs | 100 µg/mL |
| Iba1 | Goat |
Abcam ab48004 |
Dissociated mouse brain | 100 µg/mL |
| IGF2BP3 | Rabbit |
EMD Millipore 03-198 |
100 µg/mL | |
| IGFBP2 | Rabbit |
Abcam ab91404 |
(unpublished) | 100 µg/mL |
| IgG | Rabbit |
EMD Millipore AP101 |
IgG-producing cell line | 1:40 |
| Ikaros | Rabbit |
Cell Signaling Technology 14859 |
Jurkat cells | 1:10 |
| IL-18 | Rabbit |
Abcam ab207324 |
(unpublished) | 1:10 |
| IL-1beta | Goat |
R&D Systems AF-401-NA |
GM-CSF derived BMDCs | 10 µg/mL |
| IL-2Rb | Goat |
R&D Systems AF-224-NA |
PBMCs | 400 µg/mL |
| iNOS | Mouse |
R&D Systems MAB9502 |
U2OS cells | 1:10 |
| Insulin | Guinea Pig |
EMD Millipore AB3440 |
(unpublished) | 1:20 |
| Insulin* | Guinea Pig |
Dako A0564 |
(unpublished) | 1:40 |
| IRF3 | Mouse |
Novus Biologicals NBP1-47812 |
THP1 cells | 100 µg/mL |
| IRF3 | Mouse |
Novus Biologicals NBP1-04308 |
THP1 cells | 100 µg/mL |
| IRF3* | Goat |
R&D Systems AF4019 |
THP1 cells | 100 µg/mL |
| IRF5 | Goat |
Abcam ab2932 |
THP1 cells | 100 µg/mL |
| IRF5* | Sheep |
R&D Systems AF4508 |
THP1 cells | 100 µg/mL |
| K-Ras | Mouse |
Santa Cruz Biotechnology sc-517599 |
A549 | 1:10 |
| Lamin B1 | Rabbit |
R&D Systems AF8525 |
PBMCs | 50 µg/mL |
| LANA | Rat |
Abcam ab4103 |
Treated B-cells | 100 µg/mL |