Interrogating the Brain's Clock: How Spatial Genomics Enhances SCN Research
"RNAscope spatial analysis greatly improves our research by offering high-resolution visualization of gene expression within the SCN..."
- Dr. Ruchi Komal Ph.D. , Research Fellow, Samer Hattar Lab Section on Light and Circadian Rhythms (SLCR) National Institute of Mental Health, NIH
Understanding the intricate workings of the brain's suprachiasmatic nucleus (SCN), is a challenging endeavor. Yet, with the aid of RNAscopeTM in situ hybridization (ISH) spatial omics analysis tools, one researcher has made substantial progress in unveiling the complexities of SCN neuronal structures. Here’s a look into their journey and the pivotal role RNAscope has played.
Resolution matters, context matters: RNAscope resolves biology at sub-cellular level.
"RNAscope spatial analysis greatly improves our research by offering high-resolution visualization of gene expression within the SCN," research fellow Dr. Ruchi Komal at NIMH, NIH begins, highlighting the transformative impact of the technology. RNAscope ISH’s advanced visualization capability has been instrumental in “validating discoveries from single-cell data, identifying rare cell populations, and facilitating gene co-expression analysis in situ.” These features are essential for comprehending the spatial heterogeneity and neuronal structures of the SCN.
Characterization of cellular organization patterns.
RNAscope ISH spatial omics tools have not only supported, but significantly advanced neuroscience research objectives. "RNAscope products have been instrumental in facilitating substantial progress and revelations concerning the SCN neuronal structure," Dr. Komal notes. By conducting spatial distribution analyses of neurons, they have achieved successful differentiation within the SCN, identifying classical shell and core areas, alongside newly discovered subtypes. "This refined categorization contributes significantly to our comprehension of SCN complexity and organizational patterns," they add, underscoring the depth of insights gained through RNAscope.
Beyond the technical benefits, the overall satisfaction with RNAscope ISH solutions and Advanced Cell Diagnostics’ support team is remarkable: "I am amazed and very satisfied with your solutions and the support provided," Dr. Komal shares. This endorsement speaks volumes about the reliability and effectiveness of RNAscope ISH technology, as well as the quality of support that accompanies the technology.
In summary, RNAscope ISH spatial omics analysis tools have proven to be invaluable in advancing the understanding of the SCN's intricate neuronal structure. Through high-resolution visualization, validation of single-cell data, and identification of rare cell populations, RNAscope ISH has enabled significant discoveries and progress in the field. Dr. Komal’s experience is a testament to the unique value and transformative potential of RNAscope ISH spatial omics technology in the field of neuroscience research.