Dr. Zhong & Colleagues Understand Heterogeneity in Metastatic Cell Populations with Milo
Single-Cell Western
Milo permits study of proteins at the single-cell level to understand the heterogeneity among metastatic cells within a population.
Testimonials and Interviews from your Peers
Read testimonials and interviews from your peers, to find out how scientists like you are driving pioneering research and diagnostics forward using Bio-Techne reagents, kits and instruments.
Our testimonials cover a range of research areas and applications, using everything from gold standard to GMP reagents, and revolutionary, award-winning instruments.
Simply filter in the left-hand menu and see what your peers have to say.
Guts and Glory: Validating a Neuroepithelial Circuit using Milo
Single-Cell Western
We have been pairing Milo with single-cell real time qPCR data. We have the RNA and we know the transcripts are present. We used Milo to confirm our results and quantify the amount of protein that is in each individual cell.
Noelle Changes Her Brain Receptor Research Game with Wes
Simple Western
One of the best advantages of using Wes is that it saves a lot of time, both in terms of hands-on time and in time to completion of the experiment. We were spending more than 4 hours of hands-on time per Western blot with traditional methods, and we can set up a run on the Wes in about 45 minutes. Our Wes runs are completed in less than 4 hours, as opposed to 4 days with traditional methods.
Mary Ups Her Data Point Count on Laser Capture Microdissection Samples with Wes
Simple Western
We collect samples from laser microdissection and were using our entire sample on just one regular Western blot. With Wes, we can do multiple assays with one sample collection.
Miranda Sets a New Pace for Alzheimer's Research with Wes
Simple Western
The rapid, reproducible results on small amounts of tissue/cell lysates have allowed me to generate a more thorough data set on additional proteins, samples, conditions and brain regions all in the same amount, or even less time, as traditional Western blot. This technology has made it possible for small research labs to compete with the pace that research is conducted in large labs or companies.
Brian Cuts Time to Results and Solves His Membrane Protein Challenges with Wes
Simple Western
We had been trying to study receptor proteins with traditional Westerns but it was frustrating—we just could not get it. A few months with Wes and we've already had way more success than we had in three years.
Jamie Keeps Things Greener with Simple Western
Simple Western
The traditional blotting method can use up to 300 pieces of plastic and [Simple Western] uses two. What's even better is that [Simple Western] can run up to 40 proteins in three days. It would take about a week to run 40 proteins using Western blotting.
Distinguish PKG Antibody Isoforms with Simple Western
Simple Western
The Simple Western Charge assay, a capillary isoelectric focusing immunoassay, exceeded the reliability of 2D Western blots for resolving recombinant PKG-Iα and PKG-Iβ and uses 100,000X less sample quantity, making it well-suited for clinical disease proteomics because protein isoforms and post-translational modifications can be detected in precious tissue biopsies.
Emma Gets to Better Outcomes for Osteoarthritis Patients with Wes
Simple Western
Wes gives me more throughput so I can get more replicates and trust my results a little bit more, and I can trust them straight away rather than having to do a lot of repeats. And obviously I like getting results the same day rather than having to wait two days
Far Western Blot With Simple Western To Detect Protein Phosphorylation
Simple Western
The no-gel, no-transfer, no-membrane features are beneficial for the reproducibility of the assay. I really like this system over traditional Western.
Melyssa Speeds Up the Core with Wes
Simple Western
We’ve been able to increase productivity dramatically with Wes. The results are much better too, given that it removes the blotting step all together.
Make Quick Advances in Cancer Research with Simple Western
Simple Western
[Simple Western] is easy to use, convenient, and gives answers quickly. The throughput and system automation is a definite advantage.