Webinar: Novel LC-MS-Compatible icIEF Fractionation for Characterization of Innovator and Biosimilar mAbs

Discover a simple imaged capillary isoelectric focusing (icIEF) and LC-MS workflow that provides in-depth characterization of the charge variants of Benlysta™ (belimumab), a therapeutic monoclonal antibody (mAb) and a research grade biosimilar.

In this talk, we describe how the powerful combination of the MauriceFlex™ System and Waters BioAccord™ LC-MS can offer a fast, simple workflow that utilizes icIEF-based charge separation and fractionation followed by intact mass analysis. The talk demonstrates how leveraging these two benchtop platforms can uncover critical differences between a therapeutic mAb and its biosimilar. 

Key topics covered include:

• Development of a rapid, user-friendly workflow by combining icIEF-based charge separation and fractionation, followed by intact mass analysis
• Techniques to reveal critical differences between therapeutic mAbs and their biosimilars 
• How to enhance subunit analysis by incorporating a digestion step with the FabRICATOR® enzyme, offering deeper molecular insights to refine LC-MS workflows

This talk was presented at Festival of Biologics Basel in October 2024.

Speaker

Jean-François Bellec
Biologics Market Development Manager, Bio-Techne

Following a Master’s degree in Physiology at Pierre and Marie Curie University and an Engineering degree at the University of Technology of Compiègne and University of Waterloo, Jean-François moved to Oxford in 2013 to embark on an 11+-year journey at Bio-Techne. After a decade spent in the Technical Support Department, training and helping researchers with their analytical methods both remotely and in the field, he became in 2022 the Market Development Manager for Bio-Techne's Biologics systems. He was responsible for supporting the launch of the new MauriceFlex system in Europe and continues to drive new product development and collaborations to help the Biopharma community automating their workflow and accelerating their discovery-to-market process.