Webinar: Unraveling the Charge Heterogeneity of Biotherapeutics- icIEF Fractionation for Mass Spectrometry Characterization

In this webinar, we present a new and innovative MauriceFlex™ platform that can perform icIEF-based fractionation, in addition to routine CE analysis. The fractionation utilizes a special cartridge to perform isoelectric focusing to separate charge variants which are subsequently eluted via chemical mobilization for fraction collection. The MauriceFlex system provides a true pI-based fractionation method that is flexible and overcomes some of the deficiencies of IEX-based fractionation and direct CE-MS. The complete workflow from fractionation to mass spectrometry characterization will be illustrated.

Learning Objectives

• Review analytical methods for biotherapeutics charge variant characterization.
• Introduce a new icIEF platform for both protein charge variant profile analysis and fractionation.
• Demonstrate the flexibility in applying various mass spectrometry techniques to characterize charge variants collected from icIEF fractionation.