Overview

Inflammation is a fundamental biological process that occurs in response to injury or infection, and is characterized by an orchestrated cascade of immune cell activation and signaling molecules. Inflammation is associated with a wide range of diseases, including asthma, arthritis, cancer, obesity, heart disease, colitis, and neurological disorders. 

Detection of secreted factors and their receptors and cellular origin, as well as other biomarkers, is critical for understanding, diagnosing, and treating many inflammatory diseases. Understanding the spatial dynamics of these cellular and molecular interactions within tissues is crucial for elucidating the mechanisms of inflammatory diseases. RNAscope™ enables the mapping of intricate gene expression patterns associated with inflammatory responses. This technology not only sheds light on the cellular heterogeneity and functional states within inflamed tissues, but also opens new avenues for targeted therapeutic interventions.

Download Pushing Spatial Boundaries of Cytokine Detection Flyer

The Power of RNAscope Technology in Inflammation

  • Measure expression of soluble factors– Easily visualize secreted targets like chemokines and cytokines with high specificity.

  • Detect immune cell populations – Detect immune cell-specific marker proteins with RNA markers to identify immune sub-populations.

  • Decode inflammatory signaling pathways –  Spatially uncover alterations in cellular localization and activation in disease state by efficiently interrogating inflammatory signal pathway mediators.

  • Characterize infection and injury induced inflammatory responses – Understand the pathophysiology of infection by visualizing  viral/bacterial agents with the immune landscape.

  • Identify inflammatory signatures in autoimmune diseases- Detect specific biomarkers of immune response to indicate chronic inflammation and immune dysregulation

Research Areas

Featured Publications

spatially resolving microglial and non-microglial transcriptional phenotypes

Neuroimmune:

Spatially resolving microglial and non-microglial transcriptional phenotypes.

OSM and OSMR expressions by MHCII+ myeloid cells and inflammation-associated fibroblasts (IAF)

Autoimmune:

Inflammation-associated fibroblasts (IAF) and monocytes are associated with anti-TNF drug resistance via OSM signaling: OSM and OSMR expression by MHCII+ myeloid cells and IAFs. 

Resources

Webinar: Knowledge Resource Series - Inflammation Applications

In this webinar you will learn how to

  • Identify cell types secreting inflammatory cytokines and chemokines using the RNAscope multiplex assays;
  • Detect activation of immune cells in tissues during an inflammatory response;
  • Visualize RNA and protein targets simultaneously to identify cell-type specific gene expression.

 

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