Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24453

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, animal tissue and cell samples

Sensitivity

1.225 U/mL

Assay Range

1.225 - 490 U/mL

View all Acetylcholinesterase/ACHE Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Detection instrument: Microplate reader (optimum wavelength: 412 nm)
Assay time: 20 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 4.7

Inter-Assay Precision (Precision between assays) %CV < 9.3

Recovery for Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric)

Recovery

Average recovery rate: 104%

Scientific Data Images for Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric)

Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric)

Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric) [NBP3-24453] -

Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric) [NBP3-24453] - Assay Example

Kit Contents for Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric)

  • Microplate
  • Plate Sealer
  • Reagent 1: Lysis Buffer
  • Reagent 2: Buffer Solution
  • Reagent 3: Chromogenic Agent
  • Reagent 4: Substrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Acetylcholinesterase/ACHE

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Alternate Names

ACHE, ARACHE, N-ACHE

Gene Symbol

ACHE

Additional Acetylcholinesterase/ACHE Products

Product Documents for Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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