Adenosine Deaminase/ADA Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24456

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and animal tissue

Sensitivity

0.03 U/L

Assay Range

0.03 - 99 U/L

View all Adenosine Deaminase/ADA Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Detection instrument: Microplate reader (550 nm)
Assay time: 40 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 3

Inter-Assay Precision (Precision between assays) %CV < 6

Recovery for Adenosine Deaminase/ADA Activity Assay Kit (Colorimetric)

Recovery

Average recovery rate: 98%

Scientific Data Images for Adenosine Deaminase/ADA Activity Assay Kit (Colorimetric)

Adenosine Deaminase/ADA Activity Assay Kit (Colorimetric)

Adenosine Deaminase/ADA Activity Assay Kit (Colorimetric) [NBP3-24456] -

Adenosine Deaminase/ADA Activity Assay Kit (Colorimetric) [NBP3-24456] - Standard Reference Curve
Adenosine Deaminase/ADA Activity Assay Kit (Colorimetric)

Adenosine Deaminase/ADA Activity Assay Kit (Colorimetric) [NBP3-24456] -

Adenosine Deaminase/ADA Activity Assay Kit (Colorimetric) [NBP3-24456] - Assay Example

Kit Contents for Adenosine Deaminase/ADA Activity Assay Kit (Colorimetric)

  • Microplate
  • Plate Sealer
  • Reagent 1: Working Solution
  • Reagent 2: Chromogenic Agent
  • Reagent 3: 1 mmol/L Standard

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Adenosine Deaminase/ADA

Adenosine deaminase is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and from the turnover of nucleic acids in tissues. It irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the removal of an amine group. Inosine can then be deribosylated (removed from ribose) by another enzyme called purine nucleoside phosphorylase (PNP), converting it to hypoxanthine. Mutations in the gene for adenosine deaminase causing it to not be expressed are one cause of severe combined immunodeficiency (SCID). Mutations causing it to be overexpressed are one cause of hemolytic anemia. There is some evidence that a different allelle (ADA2) may lead to autism. There are 2 isoforms of ADA: ADA1 and ADA2. ADA1 is found in most body cells, particularly lymphocytes and macrophages, where it is present not only in the cytosol but also as the ecto- form on the cell membrane attached to a protein called CD26. ADA2 has only been found in the macrophage where it co-exists with ADA1 where the two isoforms regulate the ratio of adenosine to deoxyadenosine to potentiate the killing of parasites. ADA2 is the predominant form present in human plasma and is increased in many diseases, particularly those associated with the immune system: for example rheumatoid arthritis, psoriasis and sarcoidosis. The plasma AD2 isoform is also increased in most cancers.

Long Name

Adenosine Aminohydrolase

Alternate Names

ADA, ADA1

Gene Symbol

ADA

Additional Adenosine Deaminase/ADA Products

Product Documents for Adenosine Deaminase/ADA Activity Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Adenosine Deaminase/ADA Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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