APO-BRDU-IHC (TUNEL) Apoptosis Kit
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31164
Key Product Details
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Kit Type
Apoptosis Kit
Product Summary for APO-BRDU-IHC (TUNEL) Apoptosis Kit
The APO-BRDU-IHC™ kit is a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry.The kit contains the instructions and reagents required for measuring apoptosis in cells including; positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled anti-BrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, H2O2/Urea tablets for color generation and methyl green solution for counterstaining the cells.
Product Specifications
Application Notes
Please centrifuge the TdT Enzyme for 30 seconds, upon warming, to ensure all liquid is at the bottom of the tube. NOTE:- The positive and negative control cells supplied in the kit are from a cytospin preparation and do not need to be deparaffinized.
Kit Contents for APO-BRDU-IHC (TUNEL) Apoptosis Kit
- 200X Conjugate
- 5X-Reaction Buffer
- Biotin-BrDU mAb
- Blocking Buffer
- Br-dUTP
- DAB Tablets
- H202/Urea Tablets
- Methyl Green
- Positive & Negative Control Slides
- Proteinase K
- TdT Enzyme
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Storage of components varies. See protocol for specific instructions.
Background: APO-BRDU-IHC (TUNEL)
Alternate Names
APO BRDU, APO-BRDU, APO-BRDU-IHC, TUNEL assay kit
Additional APO-BRDU-IHC (TUNEL) Products
Product Documents for APO-BRDU-IHC (TUNEL) Apoptosis Kit
Product Specific Notices for APO-BRDU-IHC (TUNEL) Apoptosis Kit
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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